Detailed information of TA system TA198612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3075579..3076266
• Replicon: chromosome
• Accession: NZ_CP072764
• Organism: Mycobacterium tuberculosis strain CG20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: J8671_RS14495
• Protein ID: WP_003414064.1
• Coordinates: 3075871..3076266 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: J8671_RS14490
• Protein ID: WP_003414061.1
• Coordinates: 3075579..3075845 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8671_RS14465 (J8671_14465)	3071218..3072120	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
J8671_RS14470 (J8671_14470)	3072189..3072941	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
J8671_RS20870	3072968..3073105	-	138	Protein_2890	type II toxin-antitoxin system VapC family toxin	-
J8671_RS14475 (J8671_14475)	3073185..3073460	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
J8671_RS14480 (J8671_14480)	3073457..3075079	-	1623	WP_003906897.1	class I SAM-dependent DNA methyltransferase	-
J8671_RS14485 (J8671_14485)	3075166..3075582	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
J8671_RS14490 (J8671_14490)	3075579..3075845	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8671_RS14495 (J8671_14495)	3075871..3076266	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8671_RS14500 (J8671_14500)	3076263..3076532	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
J8671_RS14505 (J8671_14505)	3076542..3077636	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
J8671_RS14510 (J8671_14510)	3077633..3078052	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
J8671_RS20875	3078051..3078125	+	75	Protein_2899	hypothetical protein	-
J8671_RS14515 (J8671_14515)	3078126..3078605	-	480	WP_003414073.1	dihydrofolate reductase	-
J8671_RS14520 (J8671_14520)	3078676..3079476	-	801	WP_003911953.1	thymidylate synthase	-
J8671_RS14525 (J8671_14525)	3079632..3080369	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T198612 WP_003414064.1 NZ_CP072764:c3076266-3075871 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T198612 NZ_CP096923:c427501-427398 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT198612 WP_003414061.1 NZ_CP072764:c3075845-3075579 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT198612 NZ_CP096923:427393-427594 [Enterobacter hormaechei]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64