Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2935066..2935780 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name vapC Uniprot ID G0TQK0
Locus tag J8671_RS13665 Protein ID WP_003413460.1
Coordinates 2935340..2935780 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ20
Locus tag J8671_RS13660 Protein ID WP_003413456.1
Coordinates 2935066..2935353 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS13625 (J8671_13625) 2930488..2930733 + 246 WP_003413429.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
J8671_RS13630 (J8671_13630) 2930730..2931134 + 405 WP_003899389.1 type II toxin-antitoxin system VapC family toxin -
J8671_RS13635 (J8671_13635) 2931351..2931971 + 621 WP_003413441.1 DUF4178 domain-containing protein -
J8671_RS13640 (J8671_13640) 2931982..2932476 + 495 WP_003413444.1 DUF2617 family protein -
J8671_RS13645 (J8671_13645) 2932473..2932904 + 432 WP_003899390.1 DUF4247 domain-containing protein -
J8671_RS13650 (J8671_13650) 2932959..2933387 + 429 WP_178121172.1 DUF350 domain-containing protein -
J8671_RS13655 (J8671_13655) 2933384..2934955 + 1572 WP_003899392.1 polyamine aminopropyltransferase -
J8671_RS13660 (J8671_13660) 2935066..2935353 + 288 WP_003413456.1 antitoxin VapB41 Antitoxin
J8671_RS13665 (J8671_13665) 2935340..2935780 + 441 WP_003413460.1 type II toxin-antitoxin system VapC family toxin Toxin
J8671_RS13670 (J8671_13670) 2935801..2936556 - 756 WP_003413464.1 YebC/PmpR family DNA-binding transcriptional regulator -
J8671_RS13675 (J8671_13675) 2936689..2937285 - 597 WP_003413465.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
J8671_RS13680 (J8671_13680) 2937293..2938138 - 846 WP_003413466.1 acyl-CoA thioesterase II -
J8671_RS13685 (J8671_13685) 2938167..2939066 - 900 WP_003413468.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
J8671_RS13690 (J8671_13690) 2939194..2939868 + 675 WP_003413471.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-146)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 16026.38 Da        Isoelectric Point: 6.8599

>T198609 WP_003413460.1 NZ_CP072764:2935340-2935780 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Download         Length: 441 bp

>T198609 NZ_CP096920:c3199512-3199231 [Pseudomonas putida]
ATGCGGACGATTGAACAGACCAGCCGGTTCAAACGCGATTATAAACGCGAAGCCAAAGGCCCGCATCGTCAGACGCTGGC
GAGCGATTTCATTGCCGTCGTCACAGCCCTGGCGAATAACCAGCCTCTGGCCGAGAAGCACCGTGACCACGCCCTCACCG
GCGACTGGAAAGACCACCGGGACTGTCACATCAAGCCCGACCTGGTGTTGATTTACCGCAAGCCGGATGACGCCGTGCTG
CAACTCGTGCGTCTCGGATCGCATAGCGAGCTTGGCCTGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10453.98 Da        Isoelectric Point: 7.2797

>AT198609 WP_003413456.1 NZ_CP072764:2935066-2935353 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Download         Length: 288 bp

>AT198609 NZ_CP096920:c3199762-3199499 [Pseudomonas putida]
ATGTCCGCAAATGCTGTAGTCCGTGCCCGTATCGACGAGCACATCAAGGAAGAGGCGACCGTCGTGCTGGCAGCGATGGG
ACTCACCGTGTCTGACGCCTTCCGCATCATGTTGACCCGAGTCGCCCGCGAGAAAGCCTTGCCGTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCGCGCCGTGGCGGCTTGAAGTCGTTCGCTTCCGTCGAGGATCTGATG
GCGGATCTGAATGCGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQK0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BWB8

References