Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2873602..2874233 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag J8671_RS13390 Protein ID WP_003413174.1
Coordinates 2873856..2874233 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag J8671_RS13385 Protein ID WP_003413167.1
Coordinates 2873602..2873859 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS13355 (J8671_13355) 2869516..2869830 + 315 WP_009937839.1 hypothetical protein -
J8671_RS13360 (J8671_13360) 2870126..2871337 + 1212 WP_009936235.1 alpha/beta hydrolase family protein -
J8671_RS13365 (J8671_13365) 2871464..2872123 + 660 WP_003900846.1 LppA family lipoprotein -
J8671_RS13370 (J8671_13370) 2872120..2872782 + 663 WP_003900848.1 LppA family lipoprotein -
J8671_RS13375 (J8671_13375) 2872779..2873057 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
J8671_RS13380 (J8671_13380) 2873150..2873563 + 414 WP_003413164.1 PIN domain nuclease -
J8671_RS13385 (J8671_13385) 2873602..2873859 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
J8671_RS13390 (J8671_13390) 2873856..2874233 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
J8671_RS13395 (J8671_13395) 2874249..2874623 - 375 WP_003413177.1 hypothetical protein -
J8671_RS13400 (J8671_13400) 2874723..2875118 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
J8671_RS13405 (J8671_13405) 2875115..2875360 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
J8671_RS13410 (J8671_13410) 2875771..2876190 - 420 WP_003413190.1 A24 family peptidase -
J8671_RS13415 (J8671_13415) 2876202..2877011 - 810 WP_003413193.1 shikimate dehydrogenase -
J8671_RS13420 (J8671_13420) 2877008..2878261 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
J8671_RS13425 (J8671_13425) 2878254..2878766 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T198607 WP_003413174.1 NZ_CP072764:2873856-2874233 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T198607 NZ_CP096920:343708-344184 [Pseudomonas putida]
TTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATATCTGGAT
CAGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGTCATAACGAAAGCGGCGGTAACCAAGGCGA
TTGAAGCCCACGGCCAGTGGAAAGCGCCCCTAAGCTTGTGGCTGACGACCTTCGACAGGGCGACGCTGCGGTTTGAATCT
TTTGAACAGTTGAGGCAGACGTGGGTCACTGTGTCTGGCTGGAGTGTTGATCGCATCCCCCACTCAGAACTTAGGCCCGC
CAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGC
GAACGGGCACTCTCTACATCAAGGCCATTCTGTCGCACGCCGAGTACGACAAATGGTGCAGAAGCGATATCAGGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT198607 WP_003413167.1 NZ_CP072764:2873602-2873859 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT198607 NZ_CP096920:344226-344699 [Pseudomonas putida]
ATGAAGCGGGATAAAATTGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CATTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGACAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References