Detailed information of TA system TA198605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2859263..2859903
• Replicon: chromosome
• Accession: NZ_CP072764
• Organism: Mycobacterium tuberculosis strain CG20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: J8671_RS13295
• Protein ID: WP_003412970.1
• Coordinates: 2859263..2859682 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: J8671_RS13300
• Protein ID: WP_003412975.1
• Coordinates: 2859679..2859903 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8671_RS13265 (J8671_13265)	2854848..2855570	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
J8671_RS13270 (J8671_13270)	2856087..2856314	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
J8671_RS13275 (J8671_13275)	2856311..2856712	+	402	WP_003412963.1	PIN domain-containing protein	-
J8671_RS13280 (J8671_13280)	2856747..2857667	-	921	WP_003412965.1	restriction endonuclease	-
J8671_RS13285 (J8671_13285)	2858008..2858253	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
J8671_RS13290 (J8671_13290)	2858312..2859262	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
J8671_RS13295 (J8671_13295)	2859263..2859682	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8671_RS13300 (J8671_13300)	2859679..2859903	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
J8671_RS13305 (J8671_13305)	2859934..2862777	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
J8671_RS13310 (J8671_13310)	2862849..2863250	-	402	WP_003412981.1	hypothetical protein	-
J8671_RS13315 (J8671_13315)	2863250..2863720	-	471	WP_003899364.1	transcription antitermination factor NusB	-
J8671_RS13320 (J8671_13320)	2863723..2864286	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T198605 WP_003412970.1 NZ_CP072764:c2859682-2859263 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T198605 NZ_CP096917:c18698-18417 [Alcaligenes faecalis]
ATGCGGACGATTGATCGTTCGTCCGCGTTCAAGCGCGATTTCAAGCGCGAGGCCAAGGGACAGCACCGTGCCACGCTCGA
TGACGCGCTCAAGCCGGTGCTGCTCGCGCTGGCGACCGATCAGCCGTTGGACGCGCGCCACCGCGACCATGATCTTTCCG
GCGATTGGGCGGGCTACCGCGAGTGCCACATCAAGCCCGACCTGCTGCTGATTTACCGCAAGTCCGATTCCGACACTCTG
CGACTGGCGCGGCTTGGCTCGCATAGCGAGCTTTTCGGCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT198605 WP_003412975.1 NZ_CP072764:c2859903-2859679 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT198605 NZ_CP096917:c18954-18685 [Alcaligenes faecalis]
ATGAGCACTACAGCCGACACCTACGTCCGCGCCCGCATCGACACCAACACCAAGGAACGCGCCGCCAGCGCACTCGAAGC
AATGGGCTTGTCGATTTCCGACGCCATCCGCCTGTTGATGCTGCGCATCGCCGATGAGCACCGCTTGCCTTTTGACGTGA
AGGTGCCCAACGCCACGACCAAAAAGGCGATTGCCGAACTGGAAGCGGGCAAGGGCAAGAAGTTCGCCAGCGTGGACAAC
CTAATGGCAGACCTGCATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09