Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2260975..2261601 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag J8671_RS10525 Protein ID WP_003410075.1
Coordinates 2261203..2261601 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag J8671_RS10520 Protein ID WP_003911750.1
Coordinates 2260975..2261202 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS10510 (J8671_10510) 2259014..2259358 - 345 WP_003410065.1 ferredoxin family protein -
J8671_RS10515 (J8671_10515) 2259547..2260716 - 1170 WP_003899126.1 ATP-binding protein -
J8671_RS10520 (J8671_10520) 2260975..2261202 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J8671_RS10525 (J8671_10525) 2261203..2261601 + 399 WP_003410075.1 PIN domain nuclease Toxin
J8671_RS10530 (J8671_10530) 2261784..2262215 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
J8671_RS10535 (J8671_10535) 2262316..2262750 + 435 WP_003914358.1 DUF1398 domain-containing protein -
J8671_RS10540 (J8671_10540) 2263187..2263366 - 180 Protein_2106 hypothetical protein -
J8671_RS10545 (J8671_10545) 2263460..2264074 + 615 WP_234715470.1 IS110 family transposase -
J8671_RS10550 (J8671_10550) 2264028..2264618 + 591 WP_003899131.1 IS110 family transposase -
J8671_RS10555 (J8671_10555) 2264746..2266002 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T198599 WP_003410075.1 NZ_CP072764:2261203-2261601 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T198599 NZ_CP096915:51510-51791 [Pseudomonas aeruginosa]
ATGCGGACGATTGAACAGACCAGCCGGTTCAAGCGCGATTACAAACGCGAAGCCAAAGGCCCGCATCGTCAGACGCTGGC
GAGCGATTTCGTCGCCATTGTCACAGCCCTTGCGAATGACCAGCCGCTGGCCGAGAAGCACCGCGACCACGCGCTCAATG
GCGACTGGAAAGATCACCGGGACTGCCACATCAAGCCTGACCTGGTGCTGATTTACCGCAAGCCGGATGACGCCGTGTTG
CAACTCGTGCGTCTCGGCTCGCATAGCGAGCTTGGCCTGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT198599 WP_003911750.1 NZ_CP072764:2260975-2261202 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT198599 NZ_CP096915:51260-51523 [Pseudomonas aeruginosa]
ATGTCTGCCAATGCTGTAGTCCGTGCCCGTATCGACGAGCACATCAAGGAAGAGGCGACCGTCGTGCTGGCGGCGATGGG
GCTCACCGTGTCCGACGCCTTCCGCATCATGTTGACCCGCGTCGCCCGCGAGAAAGCCTTGCCGTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCGCGCCGTGGTGGCCTGAAGTCGTTTGCTTCCGTCGAGGACCTGATG
GCGGACCTGAATGCGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References