Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2235669..2236567 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag J8671_RS10400 Protein ID WP_003410001.1
Coordinates 2235669..2236229 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag J8671_RS10405 Protein ID WP_003410003.1
Coordinates 2236226..2236567 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS10370 (J8671_10370) 2230838..2231491 - 654 WP_003409976.1 cutinase Cfp21 -
J8671_RS10375 (J8671_10375) 2231606..2231836 + 231 WP_003409980.1 DUF167 domain-containing protein -
J8671_RS10380 (J8671_10380) 2231921..2232832 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
J8671_RS10385 (J8671_10385) 2232941..2233540 + 600 WP_003409989.1 L-lysine exporter -
J8671_RS10390 (J8671_10390) 2233956..2234384 + 429 WP_003409992.1 cellulose-binding protein -
J8671_RS10395 (J8671_10395) 2234610..2235149 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
J8671_RS10400 (J8671_10400) 2235669..2236229 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
J8671_RS10405 (J8671_10405) 2236226..2236567 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
J8671_RS10410 (J8671_10410) 2236653..2236910 + 258 WP_003410006.1 hypothetical protein -
J8671_RS10415 (J8671_10415) 2236811..2237146 - 336 WP_003410009.1 dehydrogenase -
J8671_RS10420 (J8671_10420) 2237235..2237579 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
J8671_RS10425 (J8671_10425) 2237573..2237821 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
J8671_RS10430 (J8671_10430) 2237921..2240236 - 2316 WP_003899120.1 cation transporter ATPase CptG -
J8671_RS10435 (J8671_10435) 2240233..2240505 - 273 WP_003410017.1 DUF1490 family protein -
J8671_RS10440 (J8671_10440) 2240558..2240914 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T198597 WP_003410001.1 NZ_CP072764:c2236229-2235669 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T198597 NZ_CP096913:c6043448-6043170 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT198597 WP_003410003.1 NZ_CP072764:c2236567-2236226 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT198597 NZ_CP096913:c6043158-6042853 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References