Detailed information of TA system TA198593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2197573..2198276
• Replicon: chromosome
• Accession: NZ_CP072764
• Organism: Mycobacterium tuberculosis strain CG20

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: J8671_RS10145
• Protein ID: WP_003409778.1
• Coordinates: 2197573..2197902 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: J8671_RS10150
• Protein ID: WP_003409780.1
• Coordinates: 2197899..2198276 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8671_RS10125 (J8671_10125)	2193956..2195026	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
J8671_RS10130 (J8671_10130)	2195023..2195538	+	516	WP_003409718.1	flavin reductase family protein	-
J8671_RS10135 (J8671_10135)	2195535..2196596	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
J8671_RS10140 (J8671_10140)	2196593..2197363	+	771	WP_003409775.1	SDR family oxidoreductase	-
J8671_RS10145 (J8671_10145)	2197573..2197902	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J8671_RS10150 (J8671_10150)	2197899..2198276	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
J8671_RS10155 (J8671_10155)	2198273..2198863	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
J8671_RS10160 (J8671_10160)	2198918..2200282	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
J8671_RS10165 (J8671_10165)	2200437..2200889	-	453	WP_003899095.1	lipoprotein	-
J8671_RS10170 (J8671_10170)	2200953..2201354	+	402	WP_003409869.1	hypothetical protein	-
J8671_RS10175 (J8671_10175)	2201347..2201529	-	183	WP_003409870.1	hypothetical protein	-
J8671_RS10180 (J8671_10180)	2201643..2201993	-	351	WP_003899096.1	hypothetical protein	-
J8671_RS10185 (J8671_10185)	2202004..2202906	-	903	WP_003899097.1	hypothetical protein	-
J8671_RS10190 (J8671_10190)	2202927..2203118	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T198593 WP_003409778.1 NZ_CP072764:c2197902-2197573 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T198593 NZ_CP096913:1658888-1659175 [Pseudomonas aeruginosa]
GTGCGCGTACTTGAGTGGCGGGAAGCTGCCCGCGCCGACCTGCTGGCGATTGTTGACTACATCTCGGACGACAACCCCGA
TGCCGCGCAGCGGTTGAAGGACGACATCGAGGCCAAGGCCGCGAAGCTGCCGGAGCGCCCGAAGCTCTACCGGCCTGGCC
GCGTGGCCGGCACGCGGGAGATGGTCGTGCGTGCGAACTACGTGGTGGTCTACATGGAGGACACCCGCGCGGTGTCCATC
CTGCGCGTGTTGCACGCCGCGCAGCAGTGGCCGCCGGCACGGGAGTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT198593 WP_003409780.1 NZ_CP072764:c2198276-2197899 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT198593 NZ_CP096913:1658602-1658901 [Pseudomonas aeruginosa]
ATGGCCGCACAGACTTCGATGCTGCACATCCGTGTGGACGATAAGCTCAAGGCCGATGCCGCCGAGAAGCTCGCCGGGGT
CGGCCTCACCGTTTCGGACGCCGTGCGCATCCTGCTGACCCGTGTGGTCAAGGAAGGCGCGCTGCCGGCTGGCCTCACGG
CCGACCCGGAGGCCTACGATGCCTGGTTCCGGGCGAAGGTCCAGGAGGCGCTGGCCGACACACGGCCGCCGGTGCCGCAC
CAGCAGGTCATGGACGAGGCGCAGGCCCTGATCGACAGGAAGCGGCGTGCGCGTACTTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8