Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1230665..1231296 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag J8671_RS05810 Protein ID WP_003898728.1
Coordinates 1230665..1230976 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag J8671_RS05815 Protein ID WP_003405836.1
Coordinates 1230976..1231296 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS05790 (J8671_05790) 1226146..1227570 - 1425 WP_003405805.1 class II fumarate hydratase -
J8671_RS05795 (J8671_05795) 1227601..1228689 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
J8671_RS05800 (J8671_05800) 1228745..1229389 + 645 WP_003915494.1 DUF4245 domain-containing protein -
J8671_RS05805 (J8671_05805) 1229396..1230553 - 1158 WP_003898727.1 AI-2E family transporter -
J8671_RS05810 (J8671_05810) 1230665..1230976 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
J8671_RS05815 (J8671_05815) 1230976..1231296 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
J8671_RS05820 (J8671_05820) 1231306..1232842 + 1537 Protein_1164 carboxylesterase/lipase family protein -
J8671_RS05825 (J8671_05825) 1232849..1233961 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
J8671_RS05830 (J8671_05830) 1233971..1234228 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
J8671_RS05835 (J8671_05835) 1234218..1235465 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
J8671_RS05840 (J8671_05840) 1235462..1236100 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T198583 WP_003898728.1 NZ_CP072764:c1230976-1230665 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T198583 NZ_CP096909:c5766546-5766268 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATGGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT198583 WP_003405836.1 NZ_CP072764:c1231296-1230976 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT198583 NZ_CP096909:c5766256-5765951 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References