Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 640231..640907 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag J8671_RS02870 Protein ID WP_003898500.1
Coordinates 640231..640644 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag J8671_RS02875 Protein ID WP_003402915.1
Coordinates 640641..640907 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS02840 (J8671_02840) 635576..635878 - 303 WP_003402896.1 DUF3349 domain-containing protein -
J8671_RS02845 (J8671_02845) 635938..636216 - 279 WP_003402901.1 hypothetical protein -
J8671_RS02850 (J8671_02850) 636213..637466 - 1254 WP_003911224.1 inorganic phosphate transporter -
J8671_RS02855 (J8671_02855) 637586..637972 - 387 WP_003402907.1 VOC family protein -
J8671_RS02860 (J8671_02860) 638035..638919 - 885 WP_003402909.1 SDR family oxidoreductase -
J8671_RS02865 (J8671_02865) 639015..639917 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
J8671_RS02870 (J8671_02870) 640231..640644 - 414 WP_003898500.1 type II toxin-antitoxin system VapC family toxin Toxin
J8671_RS02875 (J8671_02875) 640641..640907 - 267 WP_003402915.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J8671_RS02880 (J8671_02880) 641099..642814 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
J8671_RS02885 (J8671_02885) 642892..644496 + 1605 WP_003402920.1 amidohydrolase -
J8671_RS02890 (J8671_02890) 644493..645473 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T198567 WP_003898500.1 NZ_CP072764:c640644-640231 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T198567 NZ_CP096903:66134-66286 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT198567 WP_003402915.1 NZ_CP072764:c640907-640641 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT198567 NZ_CP096903:c66079-66022 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References