Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pezAT/zeta-ParD
Location 444223..445234 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name pezT Uniprot ID L7N5R2
Locus tag J8671_RS01915 Protein ID WP_003401859.1
Coordinates 444223..444816 (-) Length 198 a.a.

Antitoxin (Protein)


Gene name pezA Uniprot ID O53702
Locus tag J8671_RS01920 Protein ID WP_003401860.1
Coordinates 444845..445234 (-) Length 130 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS01895 (J8671_01895) 439872..441254 + 1383 WP_003898415.1 magnesium transporter -
J8671_RS01900 (J8671_01900) 441266..442300 - 1035 WP_003401856.1 class II fructose-bisphosphate aldolase -
J8671_RS01905 (J8671_01905) 442396..443079 + 684 WP_003401857.1 VTT domain-containing protein -
J8671_RS01910 (J8671_01910) 443068..444198 - 1131 WP_003401858.1 glycoside hydrolase family 76 protein -
J8671_RS01915 (J8671_01915) 444223..444816 - 594 WP_003401859.1 zeta toxin family protein Toxin
J8671_RS01920 (J8671_01920) 444845..445234 - 390 WP_003401860.1 hypothetical protein Antitoxin
J8671_RS01925 (J8671_01925) 445315..446526 - 1212 WP_003906353.1 VWA domain-containing protein -
J8671_RS01930 (J8671_01930) 446532..447134 - 603 WP_003898417.1 SRPBCC family protein -
J8671_RS01935 (J8671_01935) 447148..448044 - 897 WP_003898418.1 MoxR family ATPase -
J8671_RS01940 (J8671_01940) 448041..448634 - 594 WP_003401864.1 NTP transferase domain-containing protein -
J8671_RS01945 (J8671_01945) 448631..449386 - 756 WP_003401865.1 XdhC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(4-156)

Antitoxin

ParD-like antitoxin of type II bacterial toxin-antitoxin system

(6-106)


Sequences

Toxin        


Download         Length: 198 a.a.        Molecular weight: 21344.53 Da        Isoelectric Point: 10.5018

>T198566 WP_003401859.1 NZ_CP072764:c444816-444223 [Mycobacterium tuberculosis]
VKRLDLVAGPNGAGKSTFVALTLAPLLPGIVFVNADEIAKQRWPDDPTSHAYQAAQVAADTRARLIDLGRPFIAETVFSH
PSKLELIRTARTAGYTVVLHVLVIPEGLAVERVRHRVAAGGHDVPETKIRERHRRLAELVAQAITLADGATVYDNSRLAG
PRIVAQFSGGGIIGRACWPSWTPPPLMSRWSNRPETA

Download         Length: 594 bp

>T198566 NZ_CP096902:c4890566-4890255 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 130 a.a.        Molecular weight: 13648.21 Da        Isoelectric Point: 6.0829

>AT198566 WP_003401860.1 NZ_CP072764:c445234-444845 [Mycobacterium tuberculosis]
VPKAVDRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSNQHTASRRRVEAALAGHLPMTDLTLEEGVVFNAEI
SAAIEERLSRTNYGDVLAAQGITTVALNDAGDIVEHRPDGTSVVLAATP

Download         Length: 390 bp

>AT198566 NZ_CP096902:c4890254-4889964 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BRU8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BRT0

References