Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Rv0299-vapB/-
Location 363476..364047 Replicon chromosome
Accession NZ_CP072764
Organism Mycobacterium tuberculosis strain CG20

Toxin (Protein)


Gene name Rv0299 Uniprot ID -
Locus tag J8671_RS01585 Protein ID WP_003401560.1
Coordinates 363476..363778 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID O07227
Locus tag J8671_RS01590 Protein ID WP_003401563.1
Coordinates 363826..364047 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8671_RS01565 (J8671_01565) 358945..359748 - 804 WP_003401540.1 Stf0 family sulphotransferase -
J8671_RS01570 (J8671_01570) 359758..361155 - 1398 WP_003401544.1 sulfatase -
J8671_RS01575 (J8671_01575) 361334..363109 + 1776 WP_010886074.1 PE family protein -
J8671_RS01580 (J8671_01580) 363252..363479 + 228 WP_003401555.1 ribbon-helix-helix protein, CopG family -
J8671_RS01585 (J8671_01585) 363476..363778 + 303 WP_003401560.1 toxin-antitoxin system toxin Toxin
J8671_RS01590 (J8671_01590) 363826..364047 + 222 WP_003401563.1 type II toxin-antitoxin system antitoxin VapB2 Antitoxin
J8671_RS01595 (J8671_01595) 364044..364469 + 426 WP_003401566.1 PIN domain nuclease -
J8671_RS01600 (J8671_01600) 364605..365237 + 633 WP_003401571.1 TetR/AcrR family transcriptional regulator -
J8671_RS01605 (J8671_01605) 365234..366142 + 909 WP_003900117.1 protochlorophyllide reductase -
J8671_RS20565 366150..366740 - 591 WP_229298012.1 hypothetical protein -
J8671_RS20570 366733..366783 - 51 Protein_319 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Ribbon-helix-helix protein, CopG

(9-37)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 10442.16 Da        Isoelectric Point: 4.9609

>T198564 WP_003401560.1 NZ_CP072764:363476-363778 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Download         Length: 303 bp

>T198564 NZ_CP096902:c4368515-4368165 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8088.31 Da        Isoelectric Point: 10.7177

>AT198564 WP_003401563.1 NZ_CP072764:363826-364047 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Download         Length: 222 bp

>AT198564 NZ_CP096902:c4368760-4368509 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3H87
AlphaFold DB A0A829CG48

References