Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3141617..3142221 Replicon chromosome
Accession NZ_CP072763
Organism Mycobacterium tuberculosis strain CG21

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag J8666_RS14850 Protein ID WP_003414492.1
Coordinates 3141617..3142009 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P71622
Locus tag J8666_RS14855 Protein ID WP_003912003.1
Coordinates 3142006..3142221 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8666_RS14820 (J8666_14820) 3136767..3137555 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
J8666_RS14825 (J8666_14825) 3137889..3138434 - 546 WP_003913758.1 DUF1802 family protein -
J8666_RS14830 (J8666_14830) 3138706..3139590 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
J8666_RS14835 (J8666_14835) 3139593..3140480 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
J8666_RS14840 (J8666_14840) 3140785..3141330 - 546 WP_003899500.1 DUF1802 family protein -
J8666_RS14845 (J8666_14845) 3141327..3141596 - 270 WP_003414489.1 DUF2277 family protein -
J8666_RS14850 (J8666_14850) 3141617..3142009 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
J8666_RS14855 (J8666_14855) 3142006..3142221 - 216 WP_003912003.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J8666_RS14860 (J8666_14860) 3142268..3143017 + 750 WP_003414497.1 enoyl-CoA hydratase -
J8666_RS14865 (J8666_14865) 3143096..3144178 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
J8666_RS14870 (J8666_14870) 3144171..3145481 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
J8666_RS14875 (J8666_14875) 3145484..3146311 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
J8666_RS14880 (J8666_14880) 3146308..3147219 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T198554 WP_003414492.1 NZ_CP072763:c3142009-3141617 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T198554 NZ_CP096902:2146324-2146427 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7483.50 Da        Isoelectric Point: 4.9732

>AT198554 WP_003912003.1 NZ_CP072763:c3142221-3142006 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS

Download         Length: 216 bp

>AT198554 NZ_CP096902:c2146428-2146194 [Escherichia coli]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB P71622

References