Detailed information of TA system TA198552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3115164..3115734
• Replicon: chromosome
• Accession: NZ_CP072763
• Organism: Mycobacterium tuberculosis strain CG21

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: J8666_RS14705
• Protein ID: WP_003414166.1
• Coordinates: 3115164..3115520 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: J8666_RS14710
• Protein ID: WP_003901465.1
• Coordinates: 3115504..3115734 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8666_RS14685 (J8666_14685)	3110616..3112304	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
J8666_RS14690 (J8666_14690)	3112308..3112634	-	327	WP_003414157.1	hypothetical protein	-
J8666_RS14695 (J8666_14695)	3112807..3113394	+	588	WP_003914429.1	DUF3558 family protein	-
J8666_RS14700 (J8666_14700)	3113413..3115062	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
J8666_RS14705 (J8666_14705)	3115164..3115520	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
J8666_RS14710 (J8666_14710)	3115504..3115734	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
J8666_RS14715 (J8666_14715)	3115777..3116820	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
J8666_RS20890	3116819..3117286	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
J8666_RS14725 (J8666_14725)	3117462..3117716	-	255	WP_003917684.1	hypothetical protein	-
J8666_RS14730 (J8666_14730)	3117864..3118268	+	405	WP_009938577.1	hypothetical protein	-
J8666_RS14735 (J8666_14735)	3118265..3118456	+	192	WP_003414184.1	hypothetical protein	-
J8666_RS20895	3118673..3118933	+	261	Protein_2946	transposase	-
J8666_RS14745 (J8666_14745)	3120043..3120300	+	258	WP_003899489.1	hypothetical protein	-
J8666_RS14750 (J8666_14750)	3120405..3120716	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T198552 WP_003414166.1 NZ_CP072763:c3115520-3115164 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T198552 NZ_CP096902:1917554-1917805 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT198552 WP_003901465.1 NZ_CP072763:c3115734-3115504 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT198552 NZ_CP096902:1917802-1918056 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3