Detailed information of TA system TA198529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1766341..1766954
• Replicon: chromosome
• Accession: NZ_CP072763
• Organism: Mycobacterium tuberculosis strain CG21

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: J8666_RS08200
• Protein ID: WP_003407786.1
• Coordinates: 1766550..1766954 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: J8666_RS08195
• Protein ID: WP_009935474.1
• Coordinates: 1766341..1766544 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8666_RS08165 (J8666_08165)	1761746..1762126	+	381	WP_003916859.1	fumarate reductase subunit C	-
J8666_RS08170 (J8666_08170)	1762123..1762500	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
J8666_RS08175 (J8666_08175)	1762568..1763176	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
J8666_RS08180 (J8666_08180)	1763354..1764508	+	1155	Protein_1635	MMPL family transporter	-
J8666_RS08185 (J8666_08185)	1764518..1764964	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
J8666_RS08190 (J8666_08190)	1764999..1766288	+	1290	WP_003407781.1	threonine ammonia-lyase	-
J8666_RS08195 (J8666_08195)	1766341..1766544	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8666_RS08200 (J8666_08200)	1766550..1766954	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
J8666_RS08205 (J8666_08205)	1766971..1768713	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
J8666_RS08210 (J8666_08210)	1768706..1771003	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T198529 WP_003407786.1 NZ_CP072763:1766550-1766954 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T198529 NZ_CP096848:3131899-3132342 [Mycobacterium tuberculosis variant bovis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT198529 WP_009935474.1 NZ_CP072763:1766341-1766544 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT198529 NZ_CP096848:3131655-3131912 [Mycobacterium tuberculosis variant bovis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46