198516

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 754983..755775 Replicon chromosome
Accession NZ_CP072763
Organism Mycobacterium tuberculosis strain CG21

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag J8666_RS03470 Protein ID WP_003403386.1
Coordinates 755338..755775 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag J8666_RS03465 Protein ID WP_003403381.1
Coordinates 754983..755228 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8666_RS03435 (J8666_03435) 750003..751508 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
J8666_RS03440 (J8666_03440) 751589..752599 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
J8666_RS03445 (J8666_03445) 752987..753370 - 384 WP_003403365.1 ribonuclease VapC6 -
J8666_RS03450 (J8666_03450) 753465..753620 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
J8666_RS03455 (J8666_03455) 753696..754412 - 717 WP_010886082.1 CPBP family intramembrane metalloprotease -
J8666_RS03460 (J8666_03460) 754688..754996 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
J8666_RS03465 (J8666_03465) 754983..755228 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
J8666_RS03470 (J8666_03470) 755338..755775 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
J8666_RS03475 (J8666_03475) 755772..756026 - 255 WP_003911263.1 antitoxin VapB7 -
J8666_RS03480 (J8666_03480) 756140..758503 + 2364 WP_003898547.1 arylsulfatase AtsD -
J8666_RS03485 (J8666_03485) 758566..758892 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
J8666_RS03490 (J8666_03490) 758804..759142 + 339 WP_003403405.1 PIN domain-containing protein -
J8666_RS03495 (J8666_03495) 759139..759312 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T198516 WP_003403386.1 NZ_CP072763:c755775-755338 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T198516 NZ_CP096848:c2375083-2374766 [Mycobacterium tuberculosis variant bovis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT198516 WP_003403381.1 NZ_CP072763:c755228-754983 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT198516 NZ_CP096848:c2375295-2375080 [Mycobacterium tuberculosis variant bovis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References