Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 754688..755228 | Replicon | chromosome |
| Accession | NZ_CP072763 | ||
| Organism | Mycobacterium tuberculosis strain CG21 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | P9WII0 |
| Locus tag | J8666_RS03460 | Protein ID | WP_003403376.1 |
| Coordinates | 754688..754996 (-) | Length | 103 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G0TQE0 |
| Locus tag | J8666_RS03465 | Protein ID | WP_003403381.1 |
| Coordinates | 754983..755228 (-) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J8666_RS03435 (J8666_03435) | 750003..751508 | + | 1506 | WP_003403360.1 | carotenoid cleavage oxygenase | - |
| J8666_RS03440 (J8666_03440) | 751589..752599 | + | 1011 | WP_003911248.1 | ABC transporter ATP-binding protein | - |
| J8666_RS03445 (J8666_03445) | 752987..753370 | - | 384 | WP_003403365.1 | ribonuclease VapC6 | - |
| J8666_RS03450 (J8666_03450) | 753465..753620 | - | 156 | WP_003403368.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| J8666_RS03455 (J8666_03455) | 753696..754412 | - | 717 | WP_010886082.1 | CPBP family intramembrane metalloprotease | - |
| J8666_RS03460 (J8666_03460) | 754688..754996 | - | 309 | WP_003403376.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| J8666_RS03465 (J8666_03465) | 754983..755228 | - | 246 | WP_003403381.1 | ribbon-helix-helix protein, CopG family | Antitoxin |
| J8666_RS03470 (J8666_03470) | 755338..755775 | - | 438 | WP_003403386.1 | type II toxin-antitoxin system VapC family toxin | - |
| J8666_RS03475 (J8666_03475) | 755772..756026 | - | 255 | WP_003911263.1 | antitoxin VapB7 | - |
| J8666_RS03480 (J8666_03480) | 756140..758503 | + | 2364 | WP_003898547.1 | arylsulfatase AtsD | - |
| J8666_RS03485 (J8666_03485) | 758566..758892 | + | 327 | WP_003403401.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| J8666_RS03490 (J8666_03490) | 758804..759142 | + | 339 | WP_003403405.1 | PIN domain-containing protein | - |
| J8666_RS03495 (J8666_03495) | 759139..759312 | + | 174 | WP_003898549.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 103 a.a. Molecular weight: 11320.10 Da Isoelectric Point: 11.6554
>T198515 WP_003403376.1 NZ_CP072763:c754996-754688 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC
Download Length: 309 bp
>T198515 NZ_CP096848:c2338420-2337986 [Mycobacterium tuberculosis variant bovis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA
Antitoxin
Download Length: 82 a.a. Molecular weight: 9169.01 Da Isoelectric Point: 4.2070
>AT198515 WP_003403381.1 NZ_CP072763:c755228-754983 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R
Download Length: 246 bp
>AT198515 NZ_CP096848:c2338681-2338427 [Mycobacterium tuberculosis variant bovis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U4BSE5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G0TQE0 |