Detailed information of TA system TA198500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3847839..3848524
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF49
• Locus tag: J8672_RS18055
• Protein ID: WP_003917170.1
• Coordinates: 3848114..3848524 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J8672_RS18050
• Protein ID: WP_003912220.1
• Coordinates: 3847839..3848117 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS18030 (J8672_18030)	3843828..3845429	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
J8672_RS18035 (J8672_18035)	3845446..3846150	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
J8672_RS18040 (J8672_18040)	3846268..3846834	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
J8672_RS18045 (J8672_18045)	3846896..3847783	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
J8672_RS18050 (J8672_18050)	3847839..3848117	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J8672_RS18055 (J8672_18055)	3848114..3848524	+	411	WP_003917170.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8672_RS18060 (J8672_18060)	3848557..3850293	-	1737	WP_003418002.1	cholesterol oxidase	-
J8672_RS18065 (J8672_18065)	3850349..3851476	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
J8672_RS18070 (J8672_18070)	3851496..3853085	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14669.76 Da
• Isoelectric Point: 5.1784

>T198500 WP_003917170.1 NZ_CP072762:3848114-3848524 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVESMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T198500 NZ_CP096848:c1392135-1391842 [Mycobacterium tuberculosis variant bovis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT198500 WP_003912220.1 NZ_CP072762:3847839-3848117 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT198500 NZ_CP096848:c1392401-1392132 [Mycobacterium tuberculosis variant bovis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: A5U883