Detailed information of TA system TA198495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3204649..3205336
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: J8672_RS15250
• Protein ID: WP_003414624.1
• Coordinates: 3204893..3205336 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: J8672_RS15245
• Protein ID: WP_003414620.1
• Coordinates: 3204649..3204906 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS15225 (J8672_15225)	3199969..3200823	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
J8672_RS15230 (J8672_15230)	3200879..3202042	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
J8672_RS15235 (J8672_15235)	3202059..3203273	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
J8672_RS15240 (J8672_15240)	3203281..3204522	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
J8672_RS15245 (J8672_15245)	3204649..3204906	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
J8672_RS15250 (J8672_15250)	3204893..3205336	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8672_RS15255 (J8672_15255)	3205416..3206078	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
J8672_RS15260 (J8672_15260)	3206174..3206365	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
J8672_RS15265 (J8672_15265)	3206697..3208445	+	1749	WP_178121166.1	cytochrome c biogenesis protein DipZ	-
J8672_RS15270 (J8672_15270)	3208541..3209122	+	582	WP_003414644.1	fasciclin domain-containing protein	-
J8672_RS15275 (J8672_15275)	3209222..3209488	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T198495 WP_003414624.1 NZ_CP072762:3204893-3205336 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T198495 NZ_CP096848:1016995-1017429 [Mycobacterium tuberculosis variant bovis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT198495 WP_003414620.1 NZ_CP072762:3204649-3204906 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT198495 NZ_CP096848:1016810-1016989 [Mycobacterium tuberculosis variant bovis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0