Detailed information of TA system TA198494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3199048..3199596
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: J8672_RS15220
• Protein ID: WP_003414602.1
• Coordinates: 3199333..3199596 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: J8672_RS15215
• Protein ID: WP_003414599.1
• Coordinates: 3199048..3199329 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS15190 (J8672_15190)	3194671..3195528	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
J8672_RS15195 (J8672_15195)	3195570..3196154	-	585	WP_003899514.1	DUF1707 domain-containing protein	-
J8672_RS15200 (J8672_15200)	3196258..3196506	+	249	WP_003913411.1	antitoxin VapB23	-
J8672_RS15205 (J8672_15205)	3196503..3196883	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
J8672_RS15210 (J8672_15210)	3196965..3198776	-	1812	WP_003414596.1	penicillin-binding protein	-
J8672_RS15215 (J8672_15215)	3199048..3199329	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
J8672_RS15220 (J8672_15220)	3199333..3199596	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J8672_RS15225 (J8672_15225)	3199969..3200823	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
J8672_RS15230 (J8672_15230)	3200879..3202042	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
J8672_RS15235 (J8672_15235)	3202059..3203273	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
J8672_RS15240 (J8672_15240)	3203281..3204522	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T198494 WP_003414602.1 NZ_CP072762:3199333-3199596 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T198494 NZ_CP096848:844554-844982 [Mycobacterium tuberculosis variant bovis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT198494 WP_003414599.1 NZ_CP072762:3199048-3199329 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT198494 NZ_CP096848:844273-844530 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8