Detailed information of TA system TA198491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3131678..3132248
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: J8672_RS14875
• Protein ID: WP_003414166.1
• Coordinates: 3131678..3132034 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: J8672_RS14880
• Protein ID: WP_003901465.1
• Coordinates: 3132018..3132248 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS14855 (J8672_14855)	3127130..3128818	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
J8672_RS14860 (J8672_14860)	3128822..3129148	-	327	WP_003414157.1	hypothetical protein	-
J8672_RS14865 (J8672_14865)	3129321..3129908	+	588	WP_003914429.1	DUF3558 family protein	-
J8672_RS14870 (J8672_14870)	3129927..3131576	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
J8672_RS14875 (J8672_14875)	3131678..3132034	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
J8672_RS14880 (J8672_14880)	3132018..3132248	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
J8672_RS14885 (J8672_14885)	3132291..3133334	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
J8672_RS21060	3133333..3133800	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
J8672_RS14895 (J8672_14895)	3133976..3134230	-	255	WP_003917684.1	hypothetical protein	-
J8672_RS14900 (J8672_14900)	3134378..3134782	+	405	WP_009938577.1	hypothetical protein	-
J8672_RS14905 (J8672_14905)	3134779..3134970	+	192	WP_003414184.1	hypothetical protein	-
J8672_RS21065	3135187..3135447	+	261	Protein_2981	transposase	-
J8672_RS14915 (J8672_14915)	3136557..3136814	+	258	WP_003899489.1	hypothetical protein	-
J8672_RS14920 (J8672_14920)	3136919..3137230	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T198491 WP_003414166.1 NZ_CP072762:c3132034-3131678 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T198491 NZ_CP096848:c757903-757595 [Mycobacterium tuberculosis variant bovis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT198491 WP_003901465.1 NZ_CP072762:c3132248-3132018 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT198491 NZ_CP096848:c758135-757890 [Mycobacterium tuberculosis variant bovis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3