Detailed information of TA system TA198483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2875778..2876418
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: J8672_RS13460
• Protein ID: WP_003412970.1
• Coordinates: 2875778..2876197 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: J8672_RS13465
• Protein ID: WP_003412975.1
• Coordinates: 2876194..2876418 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS13430 (J8672_13430)	2871363..2872085	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
J8672_RS13435 (J8672_13435)	2872602..2872829	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
J8672_RS13440 (J8672_13440)	2872826..2873227	+	402	WP_003412963.1	PIN domain-containing protein	-
J8672_RS13445 (J8672_13445)	2873262..2874182	-	921	WP_003412965.1	restriction endonuclease	-
J8672_RS13450 (J8672_13450)	2874523..2874768	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
J8672_RS13455 (J8672_13455)	2874827..2875777	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
J8672_RS13460 (J8672_13460)	2875778..2876197	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8672_RS13465 (J8672_13465)	2876194..2876418	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
J8672_RS13470 (J8672_13470)	2876449..2879292	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
J8672_RS13475 (J8672_13475)	2879364..2879765	-	402	WP_003412981.1	hypothetical protein	-
J8672_RS13480 (J8672_13480)	2879765..2880235	-	471	WP_003899364.1	transcription antitermination factor NusB	-
J8672_RS13485 (J8672_13485)	2880238..2880801	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T198483 WP_003412970.1 NZ_CP072762:c2876197-2875778 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T198483 NZ_CP096848:680306-680713 [Mycobacterium tuberculosis variant bovis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT198483 WP_003412975.1 NZ_CP072762:c2876418-2876194 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT198483 NZ_CP096848:680094-680309 [Mycobacterium tuberculosis variant bovis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09