Detailed information of TA system TA198471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2197574..2198277
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: J8672_RS10135
• Protein ID: WP_003409778.1
• Coordinates: 2197574..2197903 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: J8672_RS10140
• Protein ID: WP_003409780.1
• Coordinates: 2197900..2198277 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS10115 (J8672_10115)	2193957..2195027	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
J8672_RS10120 (J8672_10120)	2195024..2195539	+	516	WP_003409718.1	flavin reductase family protein	-
J8672_RS10125 (J8672_10125)	2195536..2196597	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
J8672_RS10130 (J8672_10130)	2196594..2197364	+	771	WP_003409775.1	SDR family oxidoreductase	-
J8672_RS10135 (J8672_10135)	2197574..2197903	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J8672_RS10140 (J8672_10140)	2197900..2198277	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
J8672_RS10145 (J8672_10145)	2198274..2198864	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
J8672_RS10150 (J8672_10150)	2198919..2200283	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
J8672_RS10155 (J8672_10155)	2200438..2200890	-	453	WP_003899095.1	lipoprotein	-
J8672_RS10160 (J8672_10160)	2200954..2201355	+	402	WP_003409869.1	hypothetical protein	-
J8672_RS10165 (J8672_10165)	2201348..2201530	-	183	WP_003409870.1	hypothetical protein	-
J8672_RS10170 (J8672_10170)	2201644..2201994	-	351	WP_003899096.1	hypothetical protein	-
J8672_RS10175 (J8672_10175)	2202005..2202907	-	903	WP_003899097.1	hypothetical protein	-
J8672_RS10180 (J8672_10180)	2202928..2203119	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T198471 WP_003409778.1 NZ_CP072762:c2197903-2197574 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T198471 NZ_CP096847:3134687-3134950 [Mycobacterium tuberculosis variant bovis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT198471 WP_003409780.1 NZ_CP072762:c2198277-2197900 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT198471 NZ_CP096847:3134402-3134683 [Mycobacterium tuberculosis variant bovis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8