Detailed information of TA system TA198468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1766341..1766954
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: J8672_RS08195
• Protein ID: WP_003407786.1
• Coordinates: 1766550..1766954 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: J8672_RS08190
• Protein ID: WP_009935474.1
• Coordinates: 1766341..1766544 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS08160 (J8672_08160)	1761746..1762126	+	381	WP_003916859.1	fumarate reductase subunit C	-
J8672_RS08165 (J8672_08165)	1762123..1762500	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
J8672_RS08170 (J8672_08170)	1762568..1763176	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
J8672_RS08175 (J8672_08175)	1763354..1764508	+	1155	Protein_1634	MMPL family transporter	-
J8672_RS08180 (J8672_08180)	1764518..1764964	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
J8672_RS08185 (J8672_08185)	1764999..1766288	+	1290	WP_003407781.1	threonine ammonia-lyase	-
J8672_RS08190 (J8672_08190)	1766341..1766544	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8672_RS08195 (J8672_08195)	1766550..1766954	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
J8672_RS08200 (J8672_08200)	1766971..1768713	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
J8672_RS08205 (J8672_08205)	1768706..1771003	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T198468 WP_003407786.1 NZ_CP072762:1766550-1766954 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T198468 NZ_CP096847:c3065673-3065317 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT198468 WP_009935474.1 NZ_CP072762:1766341-1766544 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT198468 NZ_CP096847:c3065887-3065657 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46