Detailed information of TA system TA198464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1388691..1389250
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: J8672_RS06525
• Protein ID: WP_003898789.1
• Coordinates: 1388691..1388984 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: J8672_RS06530
• Protein ID: WP_003406322.1
• Coordinates: 1388981..1389250 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS06500 (J8672_06500)	1384284..1384544	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
J8672_RS06505 (J8672_06505)	1384541..1384972	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
J8672_RS06510 (J8672_06510)	1384995..1386683	-	1689	WP_003910308.1	PE family protein	-
J8672_RS06515 (J8672_06515)	1386863..1387723	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
J8672_RS06520 (J8672_06520)	1387804..1388634	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
J8672_RS06525 (J8672_06525)	1388691..1388984	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
J8672_RS06530 (J8672_06530)	1388981..1389250	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J8672_RS06535 (J8672_06535)	1389363..1393058	-	3696	WP_003898790.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
J8672_RS06540 (J8672_06540)	1393200..1393988	-	789	WP_003406325.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T198464 WP_003898789.1 NZ_CP072762:c1388984-1388691 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T198464 NZ_CP096847:c2836256-2835861 [Mycobacterium tuberculosis variant bovis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT198464 WP_003406322.1 NZ_CP072762:c1389250-1388981 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT198464 NZ_CP096847:c2836498-2836253 [Mycobacterium tuberculosis variant bovis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2