Detailed information of TA system TA198456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 755338..756026
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: J8672_RS03465
• Protein ID: WP_003403386.1
• Coordinates: 755338..755775 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O06777
• Locus tag: J8672_RS03470
• Protein ID: WP_003911263.1
• Coordinates: 755772..756026 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS03435 (J8672_03435)	751589..752599	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
J8672_RS03440 (J8672_03440)	752987..753370	-	384	WP_003403365.1	ribonuclease VapC6	-
J8672_RS03445 (J8672_03445)	753465..753620	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
J8672_RS03450 (J8672_03450)	753696..754412	-	717	WP_010886082.1	CPBP family intramembrane metalloprotease	-
J8672_RS03455 (J8672_03455)	754688..754996	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
J8672_RS03460 (J8672_03460)	754983..755228	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
J8672_RS03465 (J8672_03465)	755338..755775	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8672_RS03470 (J8672_03470)	755772..756026	-	255	WP_003911263.1	antitoxin VapB7	Antitoxin
J8672_RS03475 (J8672_03475)	756140..758503	+	2364	WP_003898547.1	arylsulfatase AtsD	-
J8672_RS03480 (J8672_03480)	758566..758892	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
J8672_RS03485 (J8672_03485)	758804..759142	+	339	WP_003403405.1	PIN domain-containing protein	-
J8672_RS03490 (J8672_03490)	759139..759312	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T198456 WP_003403386.1 NZ_CP072762:c755775-755338 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T198456 NZ_CP096847:2234407-2234805 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9450.99 Da
• Isoelectric Point: 11.7073

>AT198456 WP_003911263.1 NZ_CP072762:c756026-755772 [Mycobacterium tuberculosis]
MSMRLAHRLQILLDDECHRRITAVARERGVPVATVVREAIDRGLVSPAGRRKSAGRRLLDAADMSVPEPRELKQELEALR
ARRG

Nucleotide

• Length: 255 bp

>AT198456 NZ_CP096847:2234179-2234406 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: A0A806JQG1