Detailed information of TA system TA198453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 752987..753620
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: J8672_RS03440
• Protein ID: WP_003403365.1
• Coordinates: 752987..753370 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: J8672_RS03445
• Protein ID: WP_003403368.1
• Coordinates: 753465..753620 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS03415 (J8672_03415)	748279..748815	+	537	WP_003403341.1	50S ribosomal protein L10	-
J8672_RS03420 (J8672_03420)	748852..749244	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
J8672_RS03425 (J8672_03425)	749237..749932	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
J8672_RS03430 (J8672_03430)	750003..751508	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
J8672_RS03435 (J8672_03435)	751589..752599	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
J8672_RS03440 (J8672_03440)	752987..753370	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
J8672_RS03445 (J8672_03445)	753465..753620	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8672_RS03450 (J8672_03450)	753696..754412	-	717	WP_010886082.1	CPBP family intramembrane metalloprotease	-
J8672_RS03455 (J8672_03455)	754688..754996	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
J8672_RS03460 (J8672_03460)	754983..755228	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
J8672_RS03465 (J8672_03465)	755338..755775	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
J8672_RS03470 (J8672_03470)	755772..756026	-	255	WP_003911263.1	antitoxin VapB7	-
J8672_RS03475 (J8672_03475)	756140..758503	+	2364	WP_003898547.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T198453 WP_003403365.1 NZ_CP072762:c753370-752987 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T198453 NZ_CP096847:c2201966-2201547 [Mycobacterium tuberculosis variant bovis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT198453 WP_003403368.1 NZ_CP072762:c753620-753465 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT198453 NZ_CP096847:c2202235-2201975 [Mycobacterium tuberculosis variant bovis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8