Detailed information of TA system TA198450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 710785..711410
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQA0
• Locus tag: J8672_RS03220
• Protein ID: WP_003403218.1
• Coordinates: 711009..711410 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ36
• Locus tag: J8672_RS03215
• Protein ID: WP_003403213.1
• Coordinates: 710785..711012 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS03190 (J8672_03190)	705964..706185	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
J8672_RS03195 (J8672_03195)	706327..706932	+	606	WP_003898526.1	hypothetical protein	-
J8672_RS03200 (J8672_03200)	706951..709518	-	2568	WP_003900188.1	SEC-C metal-binding domain-containing protein	-
J8672_RS03205 (J8672_03205)	709602..710351	+	750	WP_003898528.1	hypothetical protein	-
J8672_RS03210 (J8672_03210)	710348..710590	+	243	WP_003403210.1	hypothetical protein	-
J8672_RS03215 (J8672_03215)	710785..711012	+	228	WP_003403213.1	antitoxin VapB29	Antitoxin
J8672_RS03220 (J8672_03220)	711009..711410	+	402	WP_003403218.1	type II toxin-antitoxin system ribonuclease VapC29	Toxin
J8672_RS20780	711539..711622	+	84	Protein_645	galactose-1-phosphate uridylyltransferase	-
J8672_RS20785	711641..712722	+	1082	Protein_646	galactose-1-phosphate uridylyltransferase	-
J8672_RS03235 (J8672_03235)	712719..713810	+	1092	WP_003906403.1	galactokinase	-
J8672_RS03240 (J8672_03240)	714205..715269	+	1065	WP_003898532.1	zinc ribbon domain-containing protein	-
J8672_RS03245 (J8672_03245)	715373..716320	+	948	WP_003403232.1	DUF732 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 13937.76 Da
• Isoelectric Point: 5.7441

>T198450 WP_003403218.1 NZ_CP072762:711009-711410 [Mycobacterium tuberculosis]
VTVLLDANVLIALVVAEHVHHDAAADWLMASDTGFATCPMTQGSLVRFLVRSGQSAAAARDVVSAVQCTSRHEFWPDALS
FAGVEVAGVVGHRQVTDAYLAQLARSHDGQLATLDSGLAHLHGDVAVLIPTTT

Nucleotide

• Length: 402 bp

>T198450 NZ_CP096847:c2183825-2183496 [Mycobacterium tuberculosis variant bovis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8163.17 Da
• Isoelectric Point: 5.6307

>AT198450 WP_003403213.1 NZ_CP072762:710785-711012 [Mycobacterium tuberculosis]
VRTTIDLPQDLHKQALAIARDTHRTLSETVADLMRRGLAANRPTALSSDPRTGLPLVSVGTVVTSEDVRSLEDEQ

Nucleotide

• Length: 228 bp

>AT198450 NZ_CP096847:c2184199-2183822 [Mycobacterium tuberculosis variant bovis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TQA0

Antitoxin

• AlphaFold DB: A0A7U4BSC9