Detailed information of TA system TA198449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 703247..703890
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: J8672_RS03170
• Protein ID: WP_003403187.1
• Coordinates: 703489..703890 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: J8672_RS03165
• Protein ID: WP_003403184.1
• Coordinates: 703247..703492 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS03130 (J8672_03130)	698527..699057	-	531	WP_003917320.1	two component system sensor kinase HK2	-
J8672_RS03135 (J8672_03135)	699041..699736	-	696	WP_003918099.1	two-component system response regulator TcrA	-
J8672_RS03140 (J8672_03140)	699859..700170	+	312	WP_003403164.1	hypothetical protein	-
J8672_RS03145 (J8672_03145)	700242..701192	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
J8672_RS03150 (J8672_03150)	701433..702017	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
J8672_RS03155 (J8672_03155)	702019..702729	+	711	Protein_631	IS607 family element RNA-guided endonuclease TnpB	-
J8672_RS03160 (J8672_03160)	702732..703202	+	471	WP_003898523.1	hypothetical protein	-
J8672_RS03165 (J8672_03165)	703247..703492	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8672_RS03170 (J8672_03170)	703489..703890	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8672_RS20775	703881..704060	+	180	Protein_635	hypothetical protein	-
J8672_RS03180 (J8672_03180)	704478..704675	-	198	WP_003403191.1	hypothetical protein	-
J8672_RS03185 (J8672_03185)	704755..705912	-	1158	WP_003898524.1	hypothetical protein	-
J8672_RS03190 (J8672_03190)	705964..706185	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
J8672_RS03195 (J8672_03195)	706327..706932	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T198449 WP_003403187.1 NZ_CP072762:703489-703890 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T198449 NZ_CP096847:1950638-1950886 [Mycobacterium tuberculosis variant bovis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT198449 WP_003403184.1 NZ_CP072762:703247-703492 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT198449 NZ_CP096847:1950426-1950638 [Mycobacterium tuberculosis variant bovis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91