Detailed information of TA system TA198448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697157..697803
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: J8672_RS03115
• Protein ID: WP_003403137.1
• Coordinates: 697157..697570 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: J8672_RS03120
• Protein ID: WP_003403139.1
• Coordinates: 697567..697803 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS03095 (J8672_03095)	693240..694790	+	1551	WP_003403119.1	MlaD family protein	-
J8672_RS03100 (J8672_03100)	694842..695234	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
J8672_RS03105 (J8672_03105)	695231..695488	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
J8672_RS03110 (J8672_03110)	695671..696906	-	1236	WP_003403128.1	ATP-binding protein	-
J8672_RS03115 (J8672_03115)	697157..697570	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8672_RS03120 (J8672_03120)	697567..697803	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
J8672_RS03125 (J8672_03125)	697907..698413	-	507	WP_003900973.1	two component system sensor kinase HK1	-
J8672_RS03130 (J8672_03130)	698527..699057	-	531	WP_003917320.1	two component system sensor kinase HK2	-
J8672_RS03135 (J8672_03135)	699041..699736	-	696	WP_003918099.1	two-component system response regulator TcrA	-
J8672_RS03140 (J8672_03140)	699859..700170	+	312	WP_003403164.1	hypothetical protein	-
J8672_RS03145 (J8672_03145)	700242..701192	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
J8672_RS03150 (J8672_03150)	701433..702017	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
J8672_RS03155 (J8672_03155)	702019..702729	+	711	Protein_631	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T198448 WP_003403137.1 NZ_CP072762:c697570-697157 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T198448 NZ_CP096847:c1930083-1929694 [Mycobacterium tuberculosis variant bovis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT198448 WP_003403139.1 NZ_CP072762:c697803-697567 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT198448 NZ_CP096847:c1930307-1930080 [Mycobacterium tuberculosis variant bovis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89