Detailed information of TA system TA198445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 640231..640907
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: J8672_RS02865
• Protein ID: WP_003898500.1
• Coordinates: 640231..640644 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: J8672_RS02870
• Protein ID: WP_003402915.1
• Coordinates: 640641..640907 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS02835 (J8672_02835)	635576..635878	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
J8672_RS02840 (J8672_02840)	635938..636216	-	279	WP_003402901.1	hypothetical protein	-
J8672_RS02845 (J8672_02845)	636213..637466	-	1254	WP_003911224.1	inorganic phosphate transporter	-
J8672_RS02850 (J8672_02850)	637586..637972	-	387	WP_003402907.1	VOC family protein	-
J8672_RS02855 (J8672_02855)	638035..638919	-	885	WP_003402909.1	SDR family oxidoreductase	-
J8672_RS02860 (J8672_02860)	639015..639917	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
J8672_RS02865 (J8672_02865)	640231..640644	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8672_RS02870 (J8672_02870)	640641..640907	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
J8672_RS02875 (J8672_02875)	641099..642814	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
J8672_RS02880 (J8672_02880)	642892..644496	+	1605	WP_003402920.1	amidohydrolase	-
J8672_RS02885 (J8672_02885)	644493..645473	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T198445 WP_003898500.1 NZ_CP072762:c640644-640231 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T198445 NZ_CP096847:c1576658-1576257 [Mycobacterium tuberculosis variant bovis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT198445 WP_003402915.1 NZ_CP072762:c640907-640641 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT198445 NZ_CP096847:c1576912-1576655 [Mycobacterium tuberculosis variant bovis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0