Detailed information of TA system TA198442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 363476..364047
• Replicon: chromosome
• Accession: NZ_CP072762
• Organism: Mycobacterium tuberculosis strain CG23

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: J8672_RS01580
• Protein ID: WP_003401560.1
• Coordinates: 363476..363778 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: J8672_RS01585
• Protein ID: WP_003401563.1
• Coordinates: 363826..364047 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8672_RS01560 (J8672_01560)	358945..359748	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
J8672_RS01565 (J8672_01565)	359758..361155	-	1398	WP_003401544.1	sulfatase	-
J8672_RS01570 (J8672_01570)	361334..363109	+	1776	WP_010886074.1	PE family protein	-
J8672_RS01575 (J8672_01575)	363252..363479	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
J8672_RS01580 (J8672_01580)	363476..363778	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
J8672_RS01585 (J8672_01585)	363826..364047	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
J8672_RS01590 (J8672_01590)	364044..364469	+	426	WP_003401566.1	PIN domain nuclease	-
J8672_RS01595 (J8672_01595)	364605..365237	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
J8672_RS01600 (J8672_01600)	365234..366142	+	909	WP_003900117.1	protochlorophyllide reductase	-
J8672_RS20740	366150..366740	-	591	WP_229298012.1	hypothetical protein	-
J8672_RS20745	366733..366783	-	51	Protein_318	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T198442 WP_003401560.1 NZ_CP072762:363476-363778 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T198442 NZ_CP096847:1387298-1387729 [Mycobacterium tuberculosis variant bovis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT198442 WP_003401563.1 NZ_CP072762:363826-364047 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT198442 NZ_CP096847:1387041-1387301 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48