Detailed information of TA system TA198434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3231585..3232272
• Replicon: chromosome
• Accession: NZ_CP072761
• Organism: Mycobacterium tuberculosis strain CG24

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: J8670_RS15370
• Protein ID: WP_003414624.1
• Coordinates: 3231829..3232272 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: J8670_RS15365
• Protein ID: WP_003414620.1
• Coordinates: 3231585..3231842 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8670_RS15345 (J8670_15345)	3226905..3227759	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
J8670_RS15350 (J8670_15350)	3227815..3228978	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
J8670_RS15355 (J8670_15355)	3228995..3230209	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
J8670_RS15360 (J8670_15360)	3230217..3231458	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
J8670_RS15365 (J8670_15365)	3231585..3231842	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
J8670_RS15370 (J8670_15370)	3231829..3232272	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8670_RS15375 (J8670_15375)	3232352..3233014	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
J8670_RS15380 (J8670_15380)	3233110..3233301	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
J8670_RS15385 (J8670_15385)	3233633..3235381	+	1749	WP_178121166.1	cytochrome c biogenesis protein DipZ	-
J8670_RS15390 (J8670_15390)	3235477..3236058	+	582	WP_003414644.1	fasciclin domain-containing protein	-
J8670_RS15395 (J8670_15395)	3236158..3236424	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T198434 WP_003414624.1 NZ_CP072761:3231829-3232272 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T198434 NZ_CP096847:c757795-757487 [Mycobacterium tuberculosis variant bovis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT198434 WP_003414620.1 NZ_CP072761:3231585-3231842 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT198434 NZ_CP096847:c758027-757782 [Mycobacterium tuberculosis variant bovis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0