Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3158614..3159184 Replicon chromosome
Accession NZ_CP072761
Organism Mycobacterium tuberculosis strain CG24

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag J8670_RS14995 Protein ID WP_003414166.1
Coordinates 3158614..3158970 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag J8670_RS15000 Protein ID WP_003901465.1
Coordinates 3158954..3159184 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8670_RS14975 (J8670_14975) 3154066..3155754 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
J8670_RS14980 (J8670_14980) 3155758..3156084 - 327 WP_003414157.1 hypothetical protein -
J8670_RS14985 (J8670_14985) 3156257..3156844 + 588 WP_003914429.1 DUF3558 family protein -
J8670_RS14990 (J8670_14990) 3156863..3158512 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
J8670_RS14995 (J8670_14995) 3158614..3158970 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
J8670_RS15000 (J8670_15000) 3158954..3159184 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
J8670_RS15005 (J8670_15005) 3159227..3160270 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
J8670_RS21190 3160269..3160736 + 468 WP_003414177.1 DUF1778 domain-containing protein -
J8670_RS15015 (J8670_15015) 3160912..3161166 - 255 WP_003917684.1 hypothetical protein -
J8670_RS15020 (J8670_15020) 3161314..3161718 + 405 WP_009938577.1 hypothetical protein -
J8670_RS15025 (J8670_15025) 3161715..3161906 + 192 WP_003414184.1 hypothetical protein -
J8670_RS21195 3162123..3162383 + 261 Protein_3000 transposase -
J8670_RS15035 (J8670_15035) 3163493..3163750 + 258 WP_003899489.1 hypothetical protein -
J8670_RS15040 (J8670_15040) 3163855..3164166 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T198430 WP_003414166.1 NZ_CP072761:c3158970-3158614 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T198430 NZ_CP096847:713284-713685 [Mycobacterium tuberculosis variant bovis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT198430 WP_003901465.1 NZ_CP072761:c3159184-3158954 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT198430 NZ_CP096847:713060-713287 [Mycobacterium tuberculosis variant bovis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References