Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3118617..3119296 Replicon chromosome
Accession NZ_CP072761
Organism Mycobacterium tuberculosis strain CG24

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag J8670_RS14775 Protein ID WP_003414059.1
Coordinates 3118617..3119033 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag J8670_RS14780 Protein ID WP_003414061.1
Coordinates 3119030..3119296 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8670_RS14755 (J8670_14755) 3114669..3115571 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
J8670_RS14760 (J8670_14760) 3115640..3116392 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
J8670_RS21175 3116419..3116556 - 138 Protein_2944 type II toxin-antitoxin system VapC family toxin -
J8670_RS14765 (J8670_14765) 3116636..3116911 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
J8670_RS14770 (J8670_14770) 3116908..3118530 - 1623 WP_003906897.1 class I SAM-dependent DNA methyltransferase -
J8670_RS14775 (J8670_14775) 3118617..3119033 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
J8670_RS14780 (J8670_14780) 3119030..3119296 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J8670_RS14785 (J8670_14785) 3119322..3119717 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
J8670_RS14790 (J8670_14790) 3119714..3119983 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
J8670_RS14795 (J8670_14795) 3119993..3121087 - 1095 WP_003414068.1 restriction endonuclease subunit S -
J8670_RS14800 (J8670_14800) 3121084..3121503 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
J8670_RS21180 3121502..3121576 + 75 Protein_2953 hypothetical protein -
J8670_RS14805 (J8670_14805) 3121577..3122056 - 480 WP_003414073.1 dihydrofolate reductase -
J8670_RS14810 (J8670_14810) 3122127..3122927 - 801 WP_003911953.1 thymidylate synthase -
J8670_RS14815 (J8670_14815) 3123083..3123820 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T198428 WP_003414059.1 NZ_CP072761:c3119033-3118617 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T198428 NZ_CP096847:c699845-699432 [Mycobacterium tuberculosis variant bovis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT198428 WP_003414061.1 NZ_CP072761:c3119296-3119030 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT198428 NZ_CP096847:c700078-699842 [Mycobacterium tuberculosis variant bovis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References