Detailed information of TA system TA198422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2902714..2903354
• Replicon: chromosome
• Accession: NZ_CP072761
• Organism: Mycobacterium tuberculosis strain CG24

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: J8670_RS13585
• Protein ID: WP_003412970.1
• Coordinates: 2902714..2903133 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: J8670_RS13590
• Protein ID: WP_003412975.1
• Coordinates: 2903130..2903354 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8670_RS13555 (J8670_13555)	2898299..2899021	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
J8670_RS13560 (J8670_13560)	2899538..2899765	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
J8670_RS13565 (J8670_13565)	2899762..2900163	+	402	WP_003412963.1	PIN domain-containing protein	-
J8670_RS13570 (J8670_13570)	2900198..2901118	-	921	WP_003412965.1	restriction endonuclease	-
J8670_RS13575 (J8670_13575)	2901459..2901704	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
J8670_RS13580 (J8670_13580)	2901763..2902713	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
J8670_RS13585 (J8670_13585)	2902714..2903133	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8670_RS13590 (J8670_13590)	2903130..2903354	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
J8670_RS13595 (J8670_13595)	2903385..2906228	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
J8670_RS13600 (J8670_13600)	2906300..2906701	-	402	WP_003412981.1	hypothetical protein	-
J8670_RS13605 (J8670_13605)	2906701..2907171	-	471	WP_003899364.1	transcription antitermination factor NusB	-
J8670_RS13610 (J8670_13610)	2907174..2907737	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T198422 WP_003412970.1 NZ_CP072761:c2903133-2902714 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T198422 NZ_CP096847:364409-364711 [Mycobacterium tuberculosis variant bovis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT198422 WP_003412975.1 NZ_CP072761:c2903354-2903130 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT198422 NZ_CP096847:364185-364412 [Mycobacterium tuberculosis variant bovis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09