Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2323762..2324398 Replicon chromosome
Accession NZ_CP072761
Organism Mycobacterium tuberculosis strain CG24

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag J8670_RS10790 Protein ID WP_003410654.1
Coordinates 2323988..2324398 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag J8670_RS10785 Protein ID WP_003410651.1
Coordinates 2323762..2323995 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8670_RS21105 2319219..2319611 + 393 WP_229303073.1 metal ABC transporter permease -
J8670_RS10775 (J8670_10775) 2319612..2320016 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
J8670_RS10780 (J8670_10780) 2320100..2323684 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
J8670_RS10785 (J8670_10785) 2323762..2323995 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
J8670_RS10790 (J8670_10790) 2323988..2324398 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
J8670_RS10795 (J8670_10795) 2324382..2325473 + 1092 WP_003900454.1 precorrin-3B synthase -
J8670_RS10800 (J8670_10800) 2325483..2326109 + 627 WP_003410658.1 precorrin-8X methylmutase -
J8670_RS10805 (J8670_10805) 2326106..2327632 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
J8670_RS10810 (J8670_10810) 2327578..2328801 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T198418 WP_003410654.1 NZ_CP072761:2323988-2324398 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T198418 NZ_CP096846:3712484-3712741 [Mycobacterium tuberculosis variant bovis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT198418 WP_003410651.1 NZ_CP072761:2323762-2323995 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT198418 NZ_CP096846:3712212-3712487 [Mycobacterium tuberculosis variant bovis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References