Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2228344..2229032 Replicon chromosome
Accession NZ_CP072761
Organism Mycobacterium tuberculosis strain CG24

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag J8670_RS10345 Protein ID WP_003409958.1
Coordinates 2228344..2228763 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag J8670_RS10350 Protein ID WP_003409968.1
Coordinates 2228772..2229032 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8670_RS10325 (J8670_10325) 2223839..2224687 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
J8670_RS10330 (J8670_10330) 2224650..2226095 - 1446 WP_003899115.1 APC family permease -
J8670_RS10335 (J8670_10335) 2226274..2226960 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
J8670_RS10340 (J8670_10340) 2227151..2228119 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
J8670_RS10345 (J8670_10345) 2228344..2228763 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
J8670_RS10350 (J8670_10350) 2228772..2229032 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J8670_RS10355 (J8670_10355) 2229175..2230851 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
J8670_RS10360 (J8670_10360) 2230839..2231492 - 654 WP_003409976.1 cutinase Cfp21 -
J8670_RS10365 (J8670_10365) 2231607..2231837 + 231 WP_003409980.1 DUF167 domain-containing protein -
J8670_RS10370 (J8670_10370) 2231922..2232833 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
J8670_RS10375 (J8670_10375) 2232942..2233541 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T198413 WP_003409958.1 NZ_CP072761:c2228763-2228344 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T198413 NZ_CP096846:c3082772-3082380 [Mycobacterium tuberculosis variant bovis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT198413 WP_003409968.1 NZ_CP072761:c2229032-2228772 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT198413 NZ_CP096846:c3082984-3082769 [Mycobacterium tuberculosis variant bovis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References