Detailed information of TA system TA198377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 51191..51710
• Replicon: plasmid pR28_180
• Accession: NZ_CP072743
• Organism: Serratia fonticola strain R28

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J8M99_RS25280
• Protein ID: WP_209524156.1
• Coordinates: 51423..51710 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J8M99_RS25275
• Protein ID: WP_209524157.1
• Coordinates: 51191..51433 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8M99_RS25255 (J8M99_25245)	46663..47433	-	771	WP_218520486.1	N-6 DNA methylase	-
J8M99_RS25260 (J8M99_25250)	48025..48483	-	459	WP_218520487.1	IS200/IS605-like element IS1541C family transposase	-
J8M99_RS25265 (J8M99_25255)	48715..49686	-	972	WP_218520488.1	ParB family protein	-
J8M99_RS25270 (J8M99_25260)	49695..50888	-	1194	WP_209524158.1	AAA family ATPase	-
J8M99_RS25275 (J8M99_25265)	51191..51433	+	243	WP_209524157.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
J8M99_RS25280 (J8M99_25270)	51423..51710	+	288	WP_209524156.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J8M99_RS25285 (J8M99_25275)	51959..52771	-	813	WP_218520489.1	tyrosine-type recombinase/integrase	-
J8M99_RS25290 (J8M99_25280)	52768..53052	-	285	WP_218520490.1	hypothetical protein	-
J8M99_RS25295 (J8M99_25285)	53856..54707	-	852	WP_218520491.1	winged helix-turn-helix domain-containing protein	-
J8M99_RS25300 (J8M99_25290)	55406..55786	+	381	WP_218520492.1	helix-turn-helix domain-containing protein	-
J8M99_RS25305 (J8M99_25295)	55898..56446	+	549	WP_218520493.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..180139	180139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11009.95 Da
• Isoelectric Point: 10.3023

>T198377 WP_209524156.1 NZ_CP072743:51423-51710 [Serratia fonticola]
MTYKIDFDNAAFKEWKKLGETVRQQLKKKLAQIVEAPRVEANRLHSLPDCYKIKLRASGYRLVYKVEDETVVILVLAVGK
RERLEVYKAAATRLS

Nucleotide

• Length: 288 bp

>T198377 NZ_CP096846:c756329-756021 [Mycobacterium tuberculosis variant bovis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8740.05 Da
• Isoelectric Point: 4.3951

>AT198377 WP_209524157.1 NZ_CP072743:51191-51433 [Serratia fonticola]
MGTMNIRIDDDLKARAGVALDGLGITASEAVRLVLEYIAQNNRLPVQVALLADEDADIIELARERLANPGKRIRVTLDDL

Nucleotide

• Length: 243 bp

>AT198377 NZ_CP096846:c756561-756316 [Mycobacterium tuberculosis variant bovis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA