Detailed information of TA system TA198358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1134587..1135149
• Replicon: chromosome
• Accession: NZ_CP072742
• Organism: Serratia fonticola strain R28

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A0F7HBD7
• Locus tag: J8M99_RS05280
• Protein ID: WP_024486364.1
• Coordinates: 1134832..1135149 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: J8M99_RS05275
• Protein ID: WP_218519922.1
• Coordinates: 1134587..1134832 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8M99_RS05245 (J8M99_05240)	1130997..1131947	+	951	WP_021807820.1	choline TMA-lyase-activating enzyme	-
J8M99_RS05250 (J8M99_05245)	1131958..1132392	+	435	WP_071682423.1	BMC domain-containing protein	-
J8M99_RS05255 (J8M99_05250)	1132428..1133063	+	636	WP_094982215.1	phosphate propanoyltransferase	-
J8M99_RS05260 (J8M99_05255)	1133074..1133742	+	669	WP_094982216.1	hypothetical protein	-
J8M99_RS05265 (J8M99_05260)	1133797..1134156	+	360	WP_021179118.1	multidrug efflux SMR transporter	-
J8M99_RS05270 (J8M99_05265)	1134168..1134497	+	330	WP_021179117.1	multidrug efflux SMR transporter	-
J8M99_RS05275 (J8M99_05270)	1134587..1134832	+	246	WP_218519922.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
J8M99_RS05280 (J8M99_05275)	1134832..1135149	+	318	WP_024486364.1	CcdB family protein	Toxin
J8M99_RS05285 (J8M99_05280)	1135390..1137186	+	1797	WP_218519923.1	glycosyl hydrolase family 28 protein	-
J8M99_RS05290 (J8M99_05285)	1137288..1138358	-	1071	WP_021807815.1	LPS export ABC transporter permease LptG	-
J8M99_RS05295 (J8M99_05290)	1138358..1139452	-	1095	WP_021807814.1	LPS export ABC transporter permease LptF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-104)

Antitoxin

• Post-segregation antitoxin CcdA (9-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11652.62 Da
• Isoelectric Point: 8.0501

>T198358 WP_024486364.1 NZ_CP072742:1134832-1135149 [Serratia fonticola]
MKQFSVYKNQSKNKKAYPFFIVVQSDLLDHLSTRLVMPLALKHATNSQVKALTPVLSVDQQDYVILTSLLTTTDVKNLNA
DCLVTNAAHLRDELVAAIDLLVLGI

Nucleotide

• Length: 318 bp

>T198358 NZ_CP096845:3131580-3132023 [Mycobacterium tuberculosis variant bovis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8902.14 Da
• Isoelectric Point: 5.0644

>AT198358 WP_218519922.1 NZ_CP072742:1134587-1134832 [Serratia fonticola]
MGTVSVIKKRNTNVYLSVDLIEKAKALDLNLSATLNHALELAVKKGQRERWLAENRAGLEALNGFVEENGLFSDDADFGV
L

Nucleotide

• Length: 246 bp

>AT198358 NZ_CP096845:3131336-3131593 [Mycobacterium tuberculosis variant bovis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0F7HBD7