Detailed information of TA system TA198356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 423180..423802
• Replicon: chromosome
• Accession: NZ_CP072742
• Organism: Serratia fonticola strain R28

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: U2M374
• Locus tag: J8M99_RS02085
• Protein ID: WP_021180142.1
• Coordinates: 423599..423802 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: U2NAN4
• Locus tag: J8M99_RS02080
• Protein ID: WP_021180141.1
• Coordinates: 423180..423548 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8M99_RS02050 (J8M99_02045)	419319..419576	+	258	WP_021180135.1	type B 50S ribosomal protein L31	-
J8M99_RS02055 (J8M99_02050)	419589..419729	+	141	WP_021805462.1	type B 50S ribosomal protein L36	-
J8M99_RS02060 (J8M99_02055)	419837..420715	-	879	WP_094981188.1	zinc ABC transporter substrate-binding protein	-
J8M99_RS02065 (J8M99_02060)	420741..421598	-	858	WP_094981189.1	metal ABC transporter permease	-
J8M99_RS02070 (J8M99_02065)	421598..422290	-	693	WP_094981217.1	ABC transporter ATP-binding protein	-
J8M99_RS02075 (J8M99_02070)	422670..423023	+	354	WP_094981218.1	hypothetical protein	-
J8M99_RS02080 (J8M99_02075)	423180..423548	+	369	WP_021180141.1	Hha toxicity modulator TomB	Antitoxin
J8M99_RS02085 (J8M99_02080)	423599..423802	+	204	WP_021180142.1	HHA domain-containing protein	Toxin
J8M99_RS02090 (J8M99_02085)	423884..424810	-	927	WP_021805469.1	LysR family transcriptional regulator	-
J8M99_RS02095 (J8M99_02090)	424947..426734	+	1788	WP_094981190.1	adenine deaminase C-terminal domain-containing protein	-
J8M99_RS02105 (J8M99_02100)	427115..427429	+	315	WP_021180150.1	MGMT family protein	-
J8M99_RS02110 (J8M99_02105)	427483..427971	-	489	WP_021180151.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8099.48 Da
• Isoelectric Point: 6.9770

>T198356 WP_021180142.1 NZ_CP072742:423599-423802 [Serratia fonticola]
MTKIDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 204 bp

>T198356 NZ_CP096845:c3085318-3084926 [Mycobacterium tuberculosis variant bovis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14097.78 Da
• Isoelectric Point: 4.5140

>AT198356 WP_021180141.1 NZ_CP072742:423180-423548 [Serratia fonticola]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYMDEGDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARTFRLFSGEGVCTTMKT

Nucleotide

• Length: 369 bp

>AT198356 NZ_CP096845:c3085530-3085315 [Mycobacterium tuberculosis variant bovis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: U2M374

Antitoxin

• AlphaFold DB: U2NAN4