Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 4247..5355 Replicon plasmid pVB6171_p3
Accession NZ_CP072734
Organism Enterococcus faecium strain VB6171

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag J7X33_RS15550 Protein ID WP_000233000.1
Coordinates 4247..5116 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag J7X33_RS15555 Protein ID WP_000205227.1
Coordinates 5131..5355 (-) Length 75 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7X33_RS15525 (J7X33_15525) 392..1072 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
J7X33_RS15530 (J7X33_15530) 1165..1959 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
J7X33_RS15535 (J7X33_15535) 2052..2594 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
J7X33_RS15540 (J7X33_15540) 2591..3499 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
J7X33_RS15545 (J7X33_15545) 3532..4266 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
J7X33_RS15550 (J7X33_15550) 4247..5116 - 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
J7X33_RS15555 (J7X33_15555) 5131..5355 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
J7X33_RS15560 (J7X33_15560) 5498..7060 - 1563 WP_000136908.1 recombinase family protein -
J7X33_RS15565 (J7X33_15565) 7062..7478 - 417 WP_000323438.1 recombinase -
J7X33_RS15570 (J7X33_15570) 7621..8301 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
J7X33_RS15575 (J7X33_15575) 8499..9104 + 606 WP_000599739.1 Fic family protein -
J7X33_RS15580 (J7X33_15580) 9120..9692 + 573 WP_000170424.1 recombinase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T198354 WP_000233000.1 NZ_CP072734:c5116-4247 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T198354 NZ_CP096845:c3058363-3058007 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT198354 WP_000205227.1 NZ_CP072734:c5355-5131 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp

>AT198354 NZ_CP096845:c3058577-3058347 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB R3JIN1


Antitoxin

Source ID Structure

References