Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 156661..157798 Replicon plasmid pVB6171_p1
Accession NZ_CP072732
Organism Enterococcus faecium strain VB6171

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag J7X33_RS14665 Protein ID WP_002401483.1
Coordinates 156661..157524 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag J7X33_RS14670 Protein ID WP_000301765.1
Coordinates 157526..157798 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7X33_RS14635 (J7X33_14635) 152568..154058 - 1491 WP_002380753.1 primase C-terminal domain-containing protein -
J7X33_RS14640 (J7X33_14640) 154453..154692 - 240 WP_002380754.1 helix-turn-helix domain-containing protein -
J7X33_RS14650 (J7X33_14650) 155054..155791 - 738 WP_001038790.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
J7X33_RS16185 (J7X33_14660) 156034..156117 - 84 Protein_159 MLS leader peptide -
J7X33_RS14665 (J7X33_14665) 156661..157524 - 864 WP_002401483.1 zeta toxin family protein Toxin
J7X33_RS14670 (J7X33_14670) 157526..157798 - 273 WP_000301765.1 antitoxin Antitoxin
J7X33_RS14675 (J7X33_14675) 157815..158030 - 216 WP_002295735.1 peptide-binding protein -
J7X33_RS14680 (J7X33_14680) 158101..158997 - 897 WP_118215883.1 ParA family protein -
J7X33_RS14685 (J7X33_14685) 159100..159360 - 261 Protein_164 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
J7X33_RS14690 (J7X33_14690) 159530..160267 - 738 WP_094899423.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
J7X33_RS14695 (J7X33_14695) 160392..160487 - 96 WP_001809736.1 23S rRNA methyltransferase attenuation leader peptide -
J7X33_RS14700 (J7X33_14700) 160536..160775 - 240 WP_000635249.1 peptide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') / erm(B) - 1..213054 213054
- inside IScluster/Tn aac(6')-aph(2'') / erm(B) - 143072..164145 21073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-213)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32486.06 Da        Isoelectric Point: 6.9965

>T198353 WP_002401483.1 NZ_CP072732:c157524-156661 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T198353 NZ_CP096845:c3019111-3018716 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT198353 WP_000301765.1 NZ_CP072732:c157798-157526 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT198353 NZ_CP096845:c3018690-3018424 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References