Detailed information of TA system TA198350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 4247..5355
• Replicon: plasmid pVB6521_p3
• Accession: NZ_CP072723
• Organism: Enterococcus faecium strain VB6521

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: J7W55_RS15550
• Protein ID: WP_000233000.1
• Coordinates: 4247..5116 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: J7W55_RS15555
• Protein ID: WP_000205227.1
• Coordinates: 5131..5355 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7W55_RS15525 (J7W55_15525)	392..1072	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
J7W55_RS15530 (J7W55_15530)	1165..1959	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
J7W55_RS15535 (J7W55_15535)	2052..2594	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
J7W55_RS15540 (J7W55_15540)	2591..3499	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
J7W55_RS15545 (J7W55_15545)	3532..4266	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
J7W55_RS15550 (J7W55_15550)	4247..5116	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
J7W55_RS15555 (J7W55_15555)	5131..5355	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
J7W55_RS15560 (J7W55_15560)	5498..7060	-	1563	WP_000136908.1	recombinase family protein	-
J7W55_RS15565 (J7W55_15565)	7062..7478	-	417	WP_000323438.1	recombinase	-
J7W55_RS15570 (J7W55_15570)	7621..8301	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
J7W55_RS15575 (J7W55_15575)	8499..9104	+	606	WP_000599739.1	Fic family protein	-
J7W55_RS15580 (J7W55_15580)	9120..9692	+	573	WP_000170424.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T198350 WP_000233000.1 NZ_CP072723:c5116-4247 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T198350 NZ_CP096845:2827642-2828019 [Mycobacterium tuberculosis variant bovis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT198350 WP_000205227.1 NZ_CP072723:c5355-5131 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT198350 NZ_CP096845:2827388-2827645 [Mycobacterium tuberculosis variant bovis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: R3JIN1