Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | omega-epsilon-zeta/zeta-epsilon |
| Location | 156661..157798 | Replicon | plasmid pVB6521_p1 |
| Accession | NZ_CP072721 | ||
| Organism | Enterococcus faecium strain VB6521 | ||
Toxin (Protein)
| Gene name | zeta | Uniprot ID | - |
| Locus tag | J7W55_RS14665 | Protein ID | WP_002401483.1 |
| Coordinates | 156661..157524 (-) | Length | 288 a.a. |
Antitoxin (Protein)
| Gene name | epsilon | Uniprot ID | R2XCR7 |
| Locus tag | J7W55_RS14670 | Protein ID | WP_000301765.1 |
| Coordinates | 157526..157798 (-) | Length | 91 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J7W55_RS14635 (J7W55_14635) | 152568..154058 | - | 1491 | WP_002380753.1 | primase C-terminal domain-containing protein | - |
| J7W55_RS14640 (J7W55_14640) | 154453..154692 | - | 240 | WP_002380754.1 | helix-turn-helix domain-containing protein | - |
| J7W55_RS14650 (J7W55_14650) | 155054..155791 | - | 738 | WP_001038790.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
| J7W55_RS16185 (J7W55_14660) | 156034..156117 | - | 84 | Protein_159 | MLS leader peptide | - |
| J7W55_RS14665 (J7W55_14665) | 156661..157524 | - | 864 | WP_002401483.1 | zeta toxin family protein | Toxin |
| J7W55_RS14670 (J7W55_14670) | 157526..157798 | - | 273 | WP_000301765.1 | antitoxin | Antitoxin |
| J7W55_RS14675 (J7W55_14675) | 157815..158030 | - | 216 | WP_002295735.1 | peptide-binding protein | - |
| J7W55_RS14680 (J7W55_14680) | 158101..158997 | - | 897 | WP_118215883.1 | ParA family protein | - |
| J7W55_RS14685 (J7W55_14685) | 159100..159360 | - | 261 | Protein_164 | topoisomerase DNA-binding C4 zinc finger domain-containing protein | - |
| J7W55_RS14690 (J7W55_14690) | 159530..160267 | - | 738 | WP_094899423.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
| J7W55_RS14695 (J7W55_14695) | 160392..160487 | - | 96 | WP_001809736.1 | 23S rRNA methyltransferase attenuation leader peptide | - |
| J7W55_RS14700 (J7W55_14700) | 160536..160775 | - | 240 | WP_000635249.1 | peptide-binding protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(6')-aph(2'') / erm(B) | - | 1..213054 | 213054 | |
| - | inside | IScluster/Tn | aac(6')-aph(2'') / erm(B) | - | 143072..164145 | 21073 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 288 a.a. Molecular weight: 32486.06 Da Isoelectric Point: 6.9965
>T198349 WP_002401483.1 NZ_CP072721:c157524-156661 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL
Download Length: 864 bp
>T198349 NZ_CP096845:2826936-2827349 [Mycobacterium tuberculosis variant bovis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA
Antitoxin
Download Length: 91 a.a. Molecular weight: 10721.25 Da Isoelectric Point: 6.4676
>AT198349 WP_000301765.1 NZ_CP072721:c157798-157526 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA
Download Length: 273 bp
>AT198349 NZ_CP096845:2827388-2827645 [Mycobacterium tuberculosis variant bovis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|