Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 490422..490993 Replicon chromosome
Accession NZ_CP072720
Organism Enterococcus faecium strain VB6521

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag J7W55_RS02325 Protein ID WP_002286801.1
Coordinates 490652..490993 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag J7W55_RS02320 Protein ID WP_002323011.1
Coordinates 490422..490652 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7W55_RS02290 (J7W55_02290) 485555..486886 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
J7W55_RS02295 (J7W55_02295) 486908..487534 + 627 WP_002286816.1 cysteine hydrolase -
J7W55_RS02300 (J7W55_02300) 487717..488298 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
J7W55_RS15910 489030..489592 + 563 Protein_457 SOS response-associated peptidase family protein -
J7W55_RS02315 (J7W55_02315) 489797..490135 - 339 WP_002286804.1 hypothetical protein -
J7W55_RS02320 (J7W55_02320) 490422..490652 + 231 WP_002323011.1 hypothetical protein Antitoxin
J7W55_RS02325 (J7W55_02325) 490652..490993 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J7W55_RS02330 (J7W55_02330) 491843..492028 + 186 WP_002304207.1 hypothetical protein -
J7W55_RS02335 (J7W55_02335) 492533..492727 + 195 WP_002297028.1 hypothetical protein -
J7W55_RS02340 (J7W55_02340) 492918..493167 + 250 Protein_463 transposase -
J7W55_RS02345 (J7W55_02345) 493411..494241 - 831 WP_169042342.1 manganese catalase family protein -
J7W55_RS02350 (J7W55_02350) 494449..495783 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T198348 WP_002286801.1 NZ_CP072720:490652-490993 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T198348 NZ_CP096845:c2812812-2812393 [Mycobacterium tuberculosis variant bovis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT198348 WP_002323011.1 NZ_CP072720:490422-490652 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT198348 NZ_CP096845:c2813033-2812809 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References