Detailed information of TA system TA198346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 7661..8180
• Replicon: plasmid pVB3378_p6
• Accession: NZ_CP072713
• Organism: Enterococcus faecium strain VB3378

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: J7155_RS15615
• Protein ID: WP_000588503.1
• Coordinates: 7923..8180 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: J7155_RS15610
• Protein ID: WP_000388479.1
• Coordinates: 7661..7930 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7155_RS15575 (J7155_15550)	3257..3673	-	417	WP_182486064.1	hypothetical protein	-
J7155_RS15580 (J7155_15555)	3675..3956	-	282	WP_002347613.1	hypothetical protein	-
J7155_RS15585 (J7155_15560)	4323..4547	+	225	WP_000410576.1	hypothetical protein	-
J7155_RS15595 (J7155_15570)	5648..6328	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
J7155_RS15600 (J7155_15575)	6404..6970	+	567	Protein_7	hypothetical protein	-
J7155_RS16050	7135..7335	+	201	WP_001033544.1	4-hydroxybenzoate octaprenyltransferase	-
J7155_RS15605 (J7155_15580)	7351..7530	+	180	WP_001120991.1	hypothetical protein	-
J7155_RS15610 (J7155_15585)	7661..7930	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J7155_RS15615 (J7155_15590)	7923..8180	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
J7155_RS15620 (J7155_15595)	8639..8794	+	156	WP_002347142.1	hypothetical protein	-
J7155_RS15625 (J7155_15600)	8818..9426	+	609	WP_224752223.1	hypothetical protein	-
J7155_RS15630 (J7155_15605)	9560..10879	+	1320	WP_000202380.1	IS1380-like element IS1678 family transposase	-
J7155_RS15635 (J7155_15610)	11092..11325	+	234	WP_002296232.1	hypothetical protein	-
J7155_RS15640 (J7155_15615)	11490..12104	-	615	WP_001261742.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..16641	16641
	-	inside	IScluster/Tn	-	-	1657..10879	9222

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T198346 WP_000588503.1 NZ_CP072713:7923-8180 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T198346 NZ_CP096845:c2377256-2376939 [Mycobacterium tuberculosis variant bovis]
ATGACGCGTAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT198346 WP_000388479.1 NZ_CP072713:7661-7930 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT198346 NZ_CP096845:c2377468-2377253 [Mycobacterium tuberculosis variant bovis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7