Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 9852..10459 Replicon plasmid pVB3378_p3
Accession NZ_CP072710
Organism Enterococcus faecium strain VB3378

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag J7155_RS15050 Protein ID WP_002321032.1
Coordinates 9852..10202 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag J7155_RS15055 Protein ID WP_002287514.1
Coordinates 10196..10459 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7155_RS15025 (J7155_15000) 6138..6722 - 585 WP_002299575.1 recombinase family protein -
J7155_RS15030 (J7155_15005) 6933..7136 - 204 WP_002299573.1 hypothetical protein -
J7155_RS15035 (J7155_15010) 7146..7568 - 423 WP_002303116.1 hypothetical protein -
J7155_RS15040 (J7155_15015) 7703..8875 - 1173 WP_000195429.1 IS256-like element IS256 family transposase -
J7155_RS15045 (J7155_15020) 9214..9828 + 615 WP_010729795.1 recombinase family protein -
J7155_RS15050 (J7155_15025) 9852..10202 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J7155_RS15055 (J7155_15030) 10196..10459 - 264 WP_002287514.1 hypothetical protein Antitoxin
J7155_RS15060 (J7155_15035) 10800..10979 - 180 WP_002305761.1 hypothetical protein -
J7155_RS16030 10995..11195 - 201 WP_002330566.1 hypothetical protein -
J7155_RS15065 (J7155_15040) 11360..12067 - 708 WP_002353391.1 hypothetical protein -
J7155_RS15070 (J7155_15045) 12060..12497 - 438 WP_002296237.1 hypothetical protein -
J7155_RS15075 (J7155_15050) 12512..12763 - 252 WP_002296238.1 hypothetical protein -
J7155_RS15080 (J7155_15055) 12763..12969 - 207 WP_002296239.1 hypothetical protein -
J7155_RS15085 (J7155_15060) 13410..13670 - 261 WP_002296240.1 hypothetical protein -
J7155_RS15090 (J7155_15065) 14036..14521 - 486 WP_002296241.1 single-stranded DNA-binding protein -
J7155_RS15095 (J7155_15070) 14524..15423 - 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-III / erm(B) - 1..43862 43862


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T198344 WP_002321032.1 NZ_CP072710:c10202-9852 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T198344 NZ_CP096845:2299315-2299725 [Mycobacterium tuberculosis variant bovis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT198344 WP_002287514.1 NZ_CP072710:c10459-10196 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT198344 NZ_CP096845:2299089-2299322 [Mycobacterium tuberculosis variant bovis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References