Detailed information of TA system TA198342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 93857..94474
• Replicon: plasmid pVB3378_p1
• Accession: NZ_CP072708
• Organism: Enterococcus faecium strain VB3378

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: J7155_RS14015
• Protein ID: WP_224451579.1
• Coordinates: 93857..94009 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: J7155_RS14020
• Protein ID: WP_002305052.1
• Coordinates: 94043..94474 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7155_RS13970 (J7155_13945)	89698..90183	+	486	Protein_99	single-stranded DNA-binding protein	-
J7155_RS13975 (J7155_13950)	90205..90462	+	258	WP_002295625.1	hypothetical protein	-
J7155_RS13980 (J7155_13955)	90621..90765	-	145	Protein_101	type I toxin-antitoxin system toxin PepG1	-
J7155_RS13985 (J7155_13960)	90933..91157	+	225	WP_074399798.1	hypothetical protein	-
J7155_RS13990 (J7155_13965)	91605..91811	+	207	WP_002324499.1	hypothetical protein	-
J7155_RS13995 (J7155_13970)	91811..92062	+	252	WP_002287517.1	hypothetical protein	-
J7155_RS14000 (J7155_13975)	92078..92515	+	438	WP_002305049.1	hypothetical protein	-
J7155_RS14005 (J7155_13980)	92508..93203	+	696	WP_002305050.1	hypothetical protein	-
J7155_RS16015	93368..93568	+	201	WP_002324500.1	hypothetical protein	-
J7155_RS14010 (J7155_13985)	93584..93763	+	180	Protein_108	hypothetical protein	-
J7155_RS14015 (J7155_13990)	93857..94009	+	153	WP_224451579.1	type II toxin-antitoxin system HicA family toxin	Toxin
J7155_RS14020 (J7155_13995)	94043..94474	+	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J7155_RS14025 (J7155_14000)	94648..95601	-	954	WP_002325884.1	IS30 family transposase	-
J7155_RS14030 (J7155_14005)	95904..97178	+	1275	WP_002323715.1	MFS transporter	-
J7155_RS14035 (J7155_14010)	97232..98659	+	1428	WP_002361237.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..227172	227172
	-	flank	IS/Tn	-	-	94648..95601	953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5815.65 Da
• Isoelectric Point: 10.3560

>T198342 WP_224451579.1 NZ_CP072708:93857-94009 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Nucleotide

• Length: 153 bp

>T198342 NZ_CP096845:c2209063-2208719 [Mycobacterium tuberculosis variant bovis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT198342 WP_002305052.1 NZ_CP072708:94043-94474 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT198342 NZ_CP096845:c2209305-2209057 [Mycobacterium tuberculosis variant bovis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8