Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1814864..1815435 Replicon chromosome
Accession NZ_CP072707
Organism Enterococcus faecium strain VB3378

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag J7155_RS08670 Protein ID WP_002286801.1
Coordinates 1815094..1815435 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag J7155_RS08665 Protein ID WP_002323011.1
Coordinates 1814864..1815094 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7155_RS08635 (J7155_08615) 1809997..1811328 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
J7155_RS08640 (J7155_08620) 1811350..1811976 + 627 WP_002286816.1 cysteine hydrolase -
J7155_RS08645 (J7155_08625) 1812159..1812740 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
J7155_RS15975 1813472..1814034 + 563 Protein_1676 SOS response-associated peptidase family protein -
J7155_RS08660 (J7155_08640) 1814239..1814577 - 339 WP_002286804.1 hypothetical protein -
J7155_RS08665 (J7155_08645) 1814864..1815094 + 231 WP_002323011.1 hypothetical protein Antitoxin
J7155_RS08670 (J7155_08650) 1815094..1815435 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J7155_RS08675 (J7155_08655) 1816285..1816470 + 186 WP_002304207.1 hypothetical protein -
J7155_RS08680 (J7155_08660) 1816975..1817169 + 195 WP_002297028.1 hypothetical protein -
J7155_RS08685 (J7155_08665) 1817360..1817609 + 250 Protein_1682 transposase -
J7155_RS08690 (J7155_08670) 1817853..1818683 - 831 WP_002286789.1 manganese catalase family protein -
J7155_RS08695 (J7155_08675) 1818891..1820225 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T198340 WP_002286801.1 NZ_CP072707:1815094-1815435 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T198340 NZ_CP096845:c2200247-2199828 [Mycobacterium tuberculosis variant bovis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT198340 WP_002323011.1 NZ_CP072707:1814864-1815094 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT198340 NZ_CP096845:c2200516-2200256 [Mycobacterium tuberculosis variant bovis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References