Detailed information of TA system TA198335

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 70834..71564
• Replicon: plasmid pKBT142
• Accession: NZ_CP072693
• Organism: Bacillus thuringiensis strain IMBL-B9

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: J2N67_RS31010
• Protein ID: WP_252598089.1
• Coordinates: 70834..71406 (-)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	71468..71564 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J2N67_RS30990 (67077)	67077..67529	-	453	WP_252598082.1	CPBP family intramembrane metalloprotease	-
J2N67_RS30995 (67559)	67559..67936	-	378	WP_252598083.1	helix-turn-helix domain-containing protein	-
J2N67_RS31000 (68111)	68111..70255	-	2145	WP_252598085.1	type IA DNA topoisomerase	-
J2N67_RS31005 (70438)	70438..70743	-	306	WP_252598087.1	hypothetical protein	-
J2N67_RS31010 (70834)	70834..71406	-	573	WP_252598089.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
- (71468)	71468..71564	-	97	NuclAT_0	-	Antitoxin
- (71468)	71468..71564	-	97	NuclAT_0	-	Antitoxin
- (71468)	71468..71564	-	97	NuclAT_0	-	Antitoxin
- (71468)	71468..71564	-	97	NuclAT_0	-	Antitoxin
J2N67_RS31015 (71644)	71644..72669	-	1026	WP_252598012.1	DUF3991 and TOPRIM domain-containing protein	-
J2N67_RS31020 (72832)	72832..73383	-	552	WP_242321001.1	metal-dependent hydrolase	-
J2N67_RS31025 (73408)	73408..75258	-	1851	WP_252598016.1	ArdC-like ssDNA-binding domain-containing protein	-
J2N67_RS31030 (75291)	75291..76076	-	786	Protein_74	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..142486	142486
	-	flank	IS/Tn	-	-	76360..77553	1193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22417.67 Da
• Isoelectric Point: 9.3616

>T198335 WP_252598089.1 NZ_CP072693:c71406-70834 [Bacillus thuringiensis]
MANKDNPKFYTISTKYIDYLRETDSKVPFNKDEKHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDNDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKMETDPKYGNLMLKQYLFLKDNMDRVSNKVEKVYKDVTVQGKPSHKQKFLKGVCCD
FSKLEEKCQEYKEKDQARRIAYMRQTGRER

Nucleotide

• Length: 573 bp

>T198335 NZ_CP096845:c1928550-1928161 [Mycobacterium tuberculosis variant bovis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Nucleotide

• Length: 97 bp

>AT198335 NZ_CP072693:c71564-71468 [Bacillus thuringiensis]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Antitoxin